壳聚糖膜的制备及其对人、大鼠皮肤成纤维细胞的相容性  

Fabrication of chitosan films and their biocompatibility to human neofetus dermal and rat dermal fibroblasts

作　　者：马建标[1] 王红军[2] 何炳林[2] 陈家童[3] 

机构地区：[1]天津工业大学,天津300160 [2]南开大学高分子化学研究所,天津300071 [3]南开大学分子生物学研究所,天津300071

出　　处：《天津工业大学学报》2001年第1期1-5,共5页Journal of Tiangong University

基　　金：国家自然科学基金资助课题 !(597730 2 1 )

摘　　要：为考察壳聚糖对皮肤成纤维细胞的相容性 ,采用流延法制备了纯壳聚糖膜和含甘油的壳聚糖膜 ,并在所制备的壳聚糖膜上进行了大鼠皮肤成纤维细胞和人胎儿皮肤成纤维细胞的体外培养 .结果表明 ,壳聚糖膜在培养开始阶段会导致部分贴附细胞的破坏 ,而进一步培养会促进这些细胞的生长和增殖 .当细胞长满膜表面发生互相接触时 ,细胞产生接触抑制现象 ,不再继续增殖 .研究还发现 ,不论在标准培养板上还是在不同的壳聚糖膜上 。Some chitosan films were fabricated by casting method using glycerol as plasticity agent in dilute acetic acid solution and then characterized.Rat dermal fibrobalasts and human neofetus dermal fibroblasts were cultured in vitro to evaluate the biocompatibility of the chitosan films.The results showed that some cells cold be broken at early stage of the culture because there were too many basic amino groups in chitosan which would tightly bind the negative surface of cells.However,while the culture was continued,cells could normally grow and proliferated.It was found that new cells would be inhibited by contact to each other after they were spread all over the surface of the films.In addition,it was also observed that human neofetus dermal fibroblasts grew slower than rat dermal fibroblasts.

关 键 词：壳聚糖膜 皮肤成纤维细胞 细胞培养 相容性 皮肤损伤 植皮 

分 类 号：R622.1[医药卫生—整形外科] R644[医药卫生—外科学]