FAF1、FLIP蛋白在青海地区胃癌及胃癌前病变组织中的表达及临床意义  

THE EXPRESSION AND CLINICAL SIGNIFICANCE OF FAF1 & FLIP IN GASTRIC CANCER AND PRECANCEROUS LESIONS OF GASTRIC CANCER IN QINGHAI AREA

作　　者：马一倩[1] 范钟麟[2] 王学红[2] 郭新建[2] 

机构地区：[1]青海卫生职业技术学院 [2]青海大学附属医院

出　　处：《青海医学院学报》2014年第2期86-91,共6页Journal of Qinghai Medical College

摘　　要：目的检测FAF1、FLIP蛋白在青海地区胃癌组织、胃癌前病变粘膜组织中的表达水平并探讨其相关性。方法用免疫组织化学法检测53例胃癌患者病变组织、52例萎缩性胃炎患者胃癌前病变粘膜组织及50例健康者胃黏膜组织中FAF1、FLIP蛋白的表达水平,作对照研究。结果正常组织、胃癌前病变及胃癌组织中的FAF1蛋白表达率分别为84.00%(42/50)、53.85%,28/52)、28.30%(15/53),各组之间比较均有显著性差异(χ2=32.59,P<0.01);在不同临床特征中FAF1蛋白表达水平与胃癌组织学分化程度相关(P<0.05);胃癌前病变组织中FAF1蛋白呈中度表达(53.85%,28/52),肠上皮化生(56.41%)与异型增生(46.15%)之间表达水平无显著性差异(χ2=0.41,P>0.05)。正常组织、胃癌前病变及胃癌组织中FLIP蛋白的表达率分别为38.00%(19/50)、36.54%(19/52)、81.13%(43/53),胃癌组与其他两组比较有显著性差异(χ2=32.59,P<0.01),在不同临床特征中其表达水平与胃癌癌细胞转移、TNM分期相关(P<0.05);胃癌前病变组织中FLIP蛋白水平较低36.54%(19/52),肠上皮化生(33.33%)与异型增生(46.15%)之间表达水平无显著性差异(χ2=0.67,P>0.05);胃癌前病变及正常组织之间无显著性差异(χ2=0.02,P>0.05)。FAF1、FLIP蛋白表达无相关性(P>0.05)。结论 1)FAF1蛋白在正常组织、胃癌前病变及胃癌组织中的表达水平逐渐降低,提示胃癌的形成过程与FAF1蛋白异常低表达相关;FLIP蛋白在胃癌组织中表达水平明显高于胃癌前病变组织和正常组织,提示胃癌的发生与FLIP蛋白异常高表达相关。检测二者水平可能为早期发现、诊断胃癌提供重要依据。2)在不同临床特征中FAF1蛋白表达水平与胃癌组织学分化程度有关,FLIP蛋白表达水平与胃癌癌细胞转移、TNM分期有关。3)与平原地区胃癌组织中FAF1、FLIP表达水平对比,本研究所示青海地区水平与其无差异。4)FAF1、FLIP蛋白表达无相关性。Objective The aim is to study the expression and clinical significance of FAF1 ＆ FLIP in gastric cancer and precancerous lesions of gastric cancer in Qinghai area. Methods The study detected the protein expression level of FAF1 and FLIP of the pathological changes of 53 patients with gastric cancer and 52 cases of precancerous lesions of gastric cancer of atrophic gastritis patients by immunohistochemistry compared with 50 cases of normal gastric mucosa tissue. Results The expression rate of FAF1 in normal tissue and the tissue of gastric cancer and precancerous lesions of gastric cancer differently was 84.0% （42/50）, 53.85% （28/52） , 28.30% （ 15/53 ） , the comparison between each group has significant differenee（χ^2 =32.59,P 〈0. 01 ）. The expression rate of FLIP was 58.00% （19/50）and 36.54% （19/52）and 81.13% （43/53）. The gastric cancer group showed significant differences compared with other two groups（x2 = 32.59, P 〈 0.01 ）, and precancerous lesions of gastric cancer showed no significant difference from normal tissues（χ^2 = 0. 02 ,P 〉 0.05 ）. The expression of FAF1 was connected with stomach histological differentiation （ P 〈 0. 05 ） ; The expression of FLIP showed relation to the gastric cancer metastasis and TNM staging; FAF1 and FLIP express no correlation （ P 〉 0. 05 ）. Conclusion 1 ） The expression rate of FAF1 in normal tissue and the tissue of gastric cancer and precancerous lesions of gastric cancer is gradually reducing, and the lack of FAF1 expression in gastric cancer cells may play an important role in the differentiation, occurrence and development of gastric cancer cell. FLIP expression level in gastric cancer tissues is obviously higher than that of the normal tissue and precancerous lesions of gastric cancer tissue, which shows that the cancer＇s occurrence and transfer relate to FLIP＇s inhibition to the mediated apoptosis of Fas/FasL. 2）The expression of FAF1 and FLIP in gastric cancer tissue has no difference in different areas.

关 键 词：青海地区 FAF1 FLIP 胃癌 胃癌前病变 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]