阻抑荧光动力学法测定痕量头孢他啶  

Study on Trace Ceftazidime by Inhibitory Kinetic Fluorimetry

作　　者：杨小丽[1] 杨金香[1] 李俊波[1] 

机构地区：[1]长治医学院药学系,046000

出　　处：《长治医学院学报》2014年第3期172-174,共3页Journal of Changzhi Medical College

基　　金：山西省高等学校科技创新项目(20121101)

摘　　要：目的：建立头孢他啶的阻抑荧光动力学测定方法。方法：利用头孢他啶对罗丹明 B-KBrO3体系的荧光反猝灭作用,以559.0 nm 为激发波长,581.0 nm 为发射波长,以对应试剂空白的荧光强度为F0,计算△F=F-F0。根据△F值与溶液中头孢他啶的浓度呈正比关系,建立阻抑荧光动力学法测定头孢他啶的新方法。结果：头孢他啶的质量浓度在0.636-8.268 ng/mL 范围内与其荧光强度呈良好线性关系（R=0.9998）,检出限可达0.283 ng/mL。结论：该法简便、快速、可靠,可用于药物制剂中头孢他啶含量的测定。Objective：To develop a kinetic-fluorimetric method for the determination of ceftazidime.Methods：The method is based on the anti-fluorescence quenching effect on rhodamine B-KBrO3 .559.0 nm and 581.0 nm were chosen as the excitation and emission wavelength for measurement of the fluorescence intensity difference △F=F- F0 of blank solution and test solution,and according to positive correlation between the value of △F and the concentration of ceftazidime.A new method of anti-fluorescence quenching for the determination of ceftazidime was established.Results：The linear range of the determination is 0.636-8.268 ng/mL（R=0.9998）with the detection limit of 0.283 ng/mL.Conclusion：The method is simple,rapid and credible,which can be used for the determination of ceftazidime in pharmaceutical formulation.

关 键 词：头孢他啶 罗丹明B 阻抑 荧光动力学法 

分 类 号：R914[医药卫生—药物化学]