青蒿琥酯对实验性自身免疫性重症肌无力大鼠的免疫调节作用  

The effect of artesunate on experimental autoimmune myasthenia gravis in rats

作　　者：张新欣[1] 李晓丽[2] 庄珊 刘深[3] 李秀华[2] 李衍滨[2] 

机构地区：[1]潍坊医学院临床学院,山东潍坊261053 [2]山东大学附属千佛山医院,山东济南250014 [3]山东中医药大学,济南250014

出　　处：《济宁医学院学报》2014年第3期165-168,共4页Journal of Jining Medical University

基　　金：山东省自然基金(编号:2010ZRA01101);山东省优秀中青年科学家科研奖励基金(编号:2008BS03012)

摘　　要：目的探究青蒿琥酯对实验性自身免疫性重症肌无力(experimental autoimmune myasthenia gravis,EAMG)大鼠TNF-α、IgG2a、IgG2b的影响。方法用人工合成的大鼠来源的乙酰胆碱受体(acetylcholine receptor,AChR)α亚基97-116肽段免疫Lewis大鼠制造重症肌无力(myasthenia gravis,MG)模型,随机分为青蒿琥酯大剂量组(100mg/kg)、小剂量组(10mg/kg)和模型对照组,流式细胞仪检测不同组大鼠淋巴结单个核细胞(mononuclear cell,MNC)中TNF-α+细胞的百分比;酶联免疫吸附法(ELISA)检测EAMG大鼠血清中抗R97-116IgG2a和IgG2b抗体的水平。结果与对照组相比,青蒿琥酯小剂量组明显下调了TNF-α+的百分比(P=0.046),而大剂量组中TNF-α下调不显著,两治疗组血清中抗R97-116IgG2a和IgG2b抗体的水平下降,且小剂量组血清中抗R97-116IgG2a和IgG2b抗体的水平下降更显著。结论青蒿琥酯主要是通过下调TNF-α+细胞的数量和降低血清中抗R97-116IgG2a和IgG2b抗体水平来改善EAMG大鼠的病情。Objective To investigate the effect of artesunate on TNF-α,IgG2a,IgG2b of experimental autoim- mune myasthenia gravis （EAMG） in rats. Methods EAMG can be induced by subcutaneous injection into both hind footpads with a synthetic peptide corresponding to region 97-116 of the rat AChRα subunit （R97-116 peptide）. Artesunate was administered by gavage to EAMG rats. The percentage of TNF-α＋ cells among lymph nodes （mononuclear cell,MNC） was analyzed by flow cytometry. Anti-R97-116 peptide IgG2a and IgG2b antibodies in serum were detected by ELISA. Results On the clay of the experiment termination,the rats in 10 mg/kg artesunate-treated group exhibited less percentage of TNF-α＋ cells and lower level of anti-R97-116 IgG antibodies （P〈0.05） com- pared with rats in control group. The therapeutic effect of artesunate on EAMG rats was associated with down-reg- ulated TNF-α＋ cells （P〈0.05） and decreased anti-R97-116 IgG antibody （P〈0.01）. Conelusion Artesunate could ameliorate EAMG by down-regulating the number of TNF-α＋ cells and decreasing anti-R97-116 IgG antibody.

关 键 词：青蒿琥酯 实验性自身免疫性重症肌无力 TNF-Α IGG2A IgG2b 

分 类 号：R746.1[医药卫生—神经病学与精神病学]