围术期肺癌患者 CD4＋／CD8＋T 细胞与TGF -β、IL -4、IL -6的检测及临床意义  

Detection and significance ofC D4 ＋/CD 8 ＋T cells ratio and TGF-β,IL-4,IL-6 levels in lung cancer patients during perioperative peir od

作　　者：韩嘉晟[1] 吴华星[2] 孟德新[1] 徐爱军[1] 孟蕊[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院内镜室,哈尔滨150081 [2]哈尔滨工业大学机电学院

出　　处：《实用肿瘤学杂志》2014年第3期193-197,共5页Practical Oncology Journal

基　　金：基金项目：黑龙江省科技厅重大攻关课题（GB05CA02-14）;哈尔滨市科技创新人才（2013RFXYJ031）

摘　　要：目的：研究围术期肺癌患者CD4＋/CD8＋T细胞与TGF-β、IL-4、IL-6的含量变化及临床意义。方法分别采用流式细胞术（Flow cytometry，FCM）及酶联免疫吸附法（ELISA）检测20例肺癌患者手术前后和10例正常对照者CD4＋T、CD8＋T细胞比例及细胞因子TGF-β、IL-4和IL-6的含量。结果20例肺癌患者术前CD4＋T、CD8＋T细胞比例分别为（46．26±4．26）％、（43．15±5．18）％，术后5天分别为（44．30±4．25）％、（39．80±2．53）％；肺癌患者术前血清中TGF-β、IL-4、IL-6的含量分别为（240．51±46．37）pg/mL、（19．85±2．52） pg/mL、（129．28±33．06） pg/mL，术后5天分别为（210．79±36．94） pg/mL、（17．37±2．57） pg/mL、（107．28±27．83） pg/mL。两组之间差异均有统计学意义（ P＜0．05）。结论围术期肺癌患者的免疫状态呈先抑制后恢复，监测围术期CD4＋/CD8＋T细胞与TGF-β、IL-4、IL-6的含量变化对肿瘤患者病情监测及预后判断具有重要意义。Objective To study the changes of CD4 ＋/CD8 ＋T cells ratio and TGF -β,IL-4,IL-6 levels in lung cancer patients during the perioperative period and to analyze the clinical significance .Methods Flow cytometer and Elisa were used to analyze the CD 4 ＋/CD8 ＋T cells ratio and TGF-β,IL-4,IL-6 levels in peripheral blood sapmles from 20 patients with lung cancer during the perioperative period and 10 healthy con-trolsR. esults Before operation ,the ratios of CD4 ＋T and CD8 ＋T cells from 20 patients with lung cancer were （46.26 ±4.26）%and（43.15 ±5.18）%, respectively.On the fifth day after operation ,the ratios of CD4 ＋T and CD8 ＋T cells were（44.30 ±4.25）%and（39.80 ±2.53）%.TGF-β,IL-4 and IL-6 levels in 20lung cancer patients were（240.51 ±46.37）pg/mL,（19.85 ±2.52）pg/mL,and（129.28 ±33.06）pg/mL,respectively.On the fifth day after operation,TGF-β,IL-4 and IL-6 levels were（210.79 ±36.94）pg/mL,（17.37 ±2.57） pg/mL,and（107.28 ±27.83） pg/mL.The differences of above parameters between the preoperative period and the fifth day after operation were statistically significant （ P〈0.05） . Conclusion The immune suppression oc-curs before its recovery during perioperative period ,the changes of CD4 ＋/CD8 ＋T cells ratio and TGF-β,IL-4 and IL-6 levels have significance on monitoring the progress and prognosis of patients with tumor .

关 键 词：肺癌 细胞因子 免疫功能 

分 类 号：R735.2[医药卫生—肿瘤]