IL -6、IL -8和 IL -10与放射性肺损伤相关性研究  

Correlative study on interleukin-6,interleukin -8 and interleukin-10 with radiation-induced lung damage

作　　者：崔莹[1] 吕达[1] 乔文波[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院放疗科,哈尔滨150081

出　　处：《实用肿瘤学杂志》2014年第3期202-206,共5页Practical Oncology Journal

基　　金：基金项目：1.黑龙江省教育厅科学基金项目（11531194）;2.黑龙江省自然科学基金项目（D200913）

摘　　要：目的：观察BALB/C小鼠肺组织照射后白细胞介素-6（IL-6）、白细胞介素-8（IL-8）和白细胞介素-10（ IL-10）三种细胞因子的表达水平，探讨其与放射性肺损伤的相关性。方法雄性BALB/C鼠，体重25 g左右，随机分为照射组（R）和对照组（C ），R组：30只，C组：10只，照射组采用6MV-X照射，小鼠肺组织常规照射，总剂量为25 Gy/5次，小鼠在照射12 w后被处死，对照组在同样的条件下处理，假照射。分别取R组及C组小鼠右肺组织，经蜡块包埋、切片后，经HE染色观察小鼠肺组织病理变化，采用免疫组化方法检测R组及C组中小鼠肺组织IL-6、IL-8和IL-10的表达水平。结果免疫组化示IL-6、IL-8和IL-10三种细胞因子主要在巨噬细胞及炎细胞内表达，结果显示R组小鼠肺组织照射12 w后IL-6和IL-8的表达水平高于C组，差异有统计学意义（P＜0．05），IL-10的表达水平低于C组，差异有统计学意义（P ＜0．05）。结论小鼠肺组织受照12 W后，病理变化提示出现肺损伤，参考免疫组化结果，细胞因子IL-6、IL-8和IL-10与放射性肺损伤相关。Objective To study radiation -induced lung damage after lung ionizing radiation and the temporal and spatial release of pro -inflammatory cytokines of interleukin -6（IL-6）,interleukin-8（IL-8） and interleukin-10（IL-10）in the irradiated lung tissue.Methods BALB/C male mice weighted around 25 g were randomly divided into two groups：radiation group （ R） and control group （ C） ,with 30 mice in R group and10 mice in C group.The thorax of mice was irradiated by 6 MV X-ray with 25 Gy in 5 fractions.The mice were sac-rificed at 12 weeks post irradiation.Lung tissues were collected and embedded in paraffin .After HE staining,lung histopathological changes were detected by immunohistochemistry to detect IL -6,IL-8 and IL-10 expression in lung tissue.Results Immunohistochemistry results showed that the expressions of IL -6,IL-8 and IL-10 were mainly expressed in macrophages and inflammatory cells .The results showed that the expressions of IL -6 and IL-8 in R group were significantly higher than that in C group .IL-10 expression level was lower than C group.Conclusion After 12 weeks exposing to radiation ,cytokines of IL-6,IL-8 and IL-10 in lung tissue are associated with radiation -induced lung injury .

关 键 词：肺损伤 动物模型 白细胞介素 细胞因子 免疫组化 

分 类 号：R734.2[医药卫生—肿瘤]