新型HK-MID-Listeria生化鉴定系统的鉴定效果评估  

Evaluation of the New Biochemical Identification System- HK- MID- Listeria

作　　者：田亮[1] 卢勉飞[1] 林干[1] 陈霖熙 蔡芷荷[1] 吴清平[2] 

机构地区：[1]广东环凯微生物科技有限公司,广东广州510663 [2]广东省微生物研究所,广东广州510663

出　　处：《食品研究与开发》2014年第10期86-88,共3页Food Research and Development

基　　金：广东省财政厅项目(2011S-P1881)

摘　　要：对新型HK-MID-Listeria生化鉴定系统的鉴定性能进行评估。以传统的生化鉴定为基准方法,对分离自食品的101株李斯特氏菌野生株用HK-MID-Listeria和API-Listeria两种鉴定方法进行鉴定比对。结果表明:HK-MID-Listeria和API-Listeria两种鉴定系统分别与传统鉴定方法符合率分别为100%和93.1%;HK-MID-Listeria与API-Listeria两者的符合率为93.1%。鉴定数据经过统计学处理,HK-MID-Listeria与API-Listeria对李斯特氏菌属的鉴定结果并无显著性差异(P>0.05)。HK-MID-Listeria鉴定李斯特氏菌属具有快速,简便的特点,不需要特殊的仪器,成本低廉,更适合应用在食品卫生方面的检测。Identification performance of the new HK-MID-Listeria biochemical identification system was assessed. Based on traditional biochemical identification method, the HK-MID-Listeria identification system has been compared with that of the API-Listeria for the identification of 101 wild strains isolated from food. Results showed：the coincidence rate of the HK-MID-Listeria and the API-Listeria respectively compared with the traditional biochemical identification methods were 100%and 93.1%;The coincidence rate of the HK-MID-Listeria compared with API-Listeria the was 93.1 %. Base on the statistical analysis, there is no significant difference among two identification system （P〉0.05） in this study. The new HK-MID-Listeria biochemical identification system has a good prospect in estimating to be attributable to consumption of foods contaminated by Listeria. Because HK-MID-Listeria is a rapid, simple and economical method to identify Listeria.

关 键 词：生化鉴定系统 HK—M ID API 

分 类 号：TS207.4[轻工技术与工程—食品科学]