IgG在人肝细胞肝癌中的表达及其临床意义  

IgG Expression in Hepatocellular Carcinoma and Its Significance

作　　者：张时超[1] 黄爱全 侯娅慧 董翠婷 刘佳睿[1] 张杰[1] 牛娜[1] 

机构地区：[1]潍坊医学院,山东潍坊261053

出　　处：《中国医学创新》2014年第18期23-26,共4页Medical Innovation of China

基　　金：国家自然科学基金资助项目(81100885);山东省优秀中青年科学家奖励基金资助项目(BS2011SW051)

摘　　要：目的:研究肿瘤源性IgG在人肝细胞肝癌中的表达和分布情况及其与临床病理学指标的关系。方法:分别应用免疫组化二步法和原位杂交检测60例肝细胞肝癌组织及其周围正常肝组织中IgG蛋白和IgG mRNA的表达,分析IgG的表达水平与9项肝细胞肝癌的临床病理学指标之间的关系。结果:肝癌组织中IgG的表达明显高于其周围正常肝组织,且两者表达程度比较差异有统计学意义(P=0.001);IgG在肿瘤组织中的异常高表达与肿瘤分化程度和pTNM分期呈负相关(r=-0.357,P=0.005;r=-0.296,P=0.021),与血浆AFP浓度呈正相关(r=0.336,P=0.009),与年龄、性别、肿瘤包膜的完整性、肿瘤大小、肿瘤个数和一年内是否复发或转移无相关性(P>0.05)。结论:肝癌细胞所产生的IgG与肿瘤的分化、pTMN分期相关,可能作为辅助肝细胞肝癌病理学诊断和预后评估的一项重要指标。To investigate the expression and distribution of IgG in human liver cancer cells and its correlation with clinicopathological factors of hepatocellular carcinoma.Method：Fresh and paraffin-embedded tissues from 60 cases of HCC tumor with the matched normal liver specimens were detected by two-step immunohistochemistry and in situ hybridization for the expression of IgG protein and mRNA respectively. The relationship between cancer-derived IgG expression and 9 clinicopathological factors were analyzed statistically.Result：Signals of IgG protein in hepatocellular carcinoma cancer cells was significantly stronger than that in its corresponding normal tissues（P=0.001）;the over-expression of IgG was negatively correlated with several clinicopathological factors such as tumor differentiation grade and pTNM（r=-0.357,P=0.005;r=-0.296,P=0.021）,and positively associated with AFP concentration in serum （r=0.336,P=0.009）. However,no correlationship with age,gender,capsular infiltration,tumor size,tumor quantity and recurrence or metastasis of the disease within 1 year was found（P〉0.05）. Conclusion：Liver-cancer-derived IgG may has a profound impact on the differentiation and pTNM staging of liver cancer cell,and might be served as a tumour marker to help pathological diagnosis and evaluate the prognosis for patients.

关 键 词：IGG 肝癌 临床病理学指标 原位杂交 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]