丙泊酚、咪达唑仑和硫喷妥钠对大鼠局灶脑缺血再灌注的影响(英文)  

Effects of propofol, midazolam and thiopental sodium on outcome of focal cerebral ischemia reperfusion in rats

作　　者：陈莲华[1] 贡沁燕[2] 俞彰[3] 陈英子[1] 肖常思[1] 

机构地区：[1]复旦大学医学院附属眼耳鼻喉科医院麻醉科,上海200031 [2]复旦大学基础医学院药理教研室,上海200032 [3]复旦大学基础医学院电镜室,上海200032

出　　处：《中国新药与临床杂志》2001年第2期81-86,共6页Chinese Journal of New Drugs and Clinical Remedies

摘　　要：目的 :观察丙泊酚、咪达唑仑和硫喷妥钠对大鼠局灶脑缺血再灌注后的神经功能缺陷和病理学后果的影响。方法 :雄性SD大鼠 ,采用大脑中动脉内线栓法 (MCAO)诱发局灶脑缺血。经历 3h缺血和 2 4h再灌注后 ,按 0～ 5分级标准判断神经功能缺陷程度。经氯化三苯四唑 (TTC)染色后 ,用Im age分析系统计算脑梗死容积 ,电镜下观察梗死边缘组织的细胞超微结构。结果 :丙泊酚和咪达唑仑改善大鼠神经功能缺陷程度 ,降低脑梗死和脑水肿容积 ,并减少组织超微结构损伤。且丙泊酚的保护作用强于咪达唑仑。硫喷妥钠未显示脑保护作用。结论AIM: To investigate the effects of propofol, midazolam and thiopental sodium on neurologic and histologic outcome from focal cerebral ischemia reperfusion in a rat model of reversible middle cerebral artery occlusion (MCAO). METHODS: Male SD rats were scheduled to undergo 3 h MCAO by intraluminal suture and 24 h reperfusion. Neurologic outcome was scored with a 0-5 grading scale. Infarct volume was shown with TTC staining and measured by image analysis system. Ultrastructure of the tissues taken from the brim of the damaged area was examined under electron microscope. RESULTS: Both propofol and midazolam could attenuate neurologic deficits, reduce infarct and edema volumes, and ameliorate ultrastructure damage at the brim of lesion. Propofol showed better neuroprotection than midazolam while thiopental sodium did not exhibit protective effect. CONCLUSION: Propofol and midazolam, but not thiopental sodium, can provide protective effects against reperfusion induced injury in rats subjected to focal cerebral ischemia.

关 键 词：脑缺血 再灌注损伤 丙泊酚 咪达唑仑 硫喷妥钠 治疗 

分 类 号：R743.43[医药卫生—神经病学与精神病学] R972[医药卫生—临床医学]