腹泻症候群致泻性大肠埃希氏菌血清型及毒力基因检测  

The Bacterium Serotype and Virulence Gene Detection of Diarrhea Syndrome Caused by Diarrhea Escherichia Coli

作　　者：慕毅敏[1] 蔡旭明[1] 周敬纲 蔡旭辉[3] 

机构地区：[1]吉林省四平市疾病预防控制中心,吉林四平136000 [2]吉林省公主岭市疾病预防控制中心 [3]吉林省四平市卫生局卫生监督所

出　　处：《中国医学创新》2014年第17期125-128,共4页Medical Innovation of China

摘　　要：目的:通过对腹泻症候群中致泻大肠埃希菌的血清型和多重PCR检测,了解本地区致泻大肠埃希菌的血清群、型分布规律,所携带毒力基因及相互之间的相关性,为腹泻症候群中致泻大肠埃希菌的检测、鉴定提供科学依据,以提高阳性株的检出率。方法:对本地区2012-2013年腹泻症候群监测医院门诊未使用过抗生素腹泻患者的稀便、水样便、黏脓便或脓血便标本通过増菌、各种选择性培养基筛选出病原菌,后经生化试验、多重PCR试验和血清型分型试验进行鉴定。结果:本次检测共检出65株血清凝集致泻大肠埃希菌,分属EPEC、EIEC、ETEC,以EPEC为主,占83.08%,共8个血清型,其中血清型O55:H59、O128:H67占58.47%;共检出4种相关毒力基因,其中escV 49株(75.38%)。未分血清型菌株27株,检出相关毒力基因共5种,分属EPEC、EIEC、ETEC、EAEC,其中escV 15株(55.56%)。结论:本地区腹泻症候群中血清学阳性致泻大肠埃希菌以EPEC为主,常见血清型为O55:H59、O128:H67,毒力基因为escV、escV+bfpB、invE、elt。未分血清型致泻大肠埃希菌毒力基因为escV、escV+bfpB、invE、elt、astA,与已分型菌株携带基因基本一致。腹泻患者的大肠埃希菌检测中,在传统的细菌分离培养、生化试验、血清学鉴定的基础上,有必要进行大肠埃希菌的毒力基因检测,以提高阳性检出率,避免漏检,提高致泻性大肠埃希菌的诊断。Objective：To detect the serum type of diarrhea caused by Escherichia coli diarrhea syndrome and multiple PCR,understand the diarrhea caused by Escherichia coli serogroups,region based distribution,the correlation between virulence genes and their detection,identification,and provide scientific basis for diarrhea syndrome caused by large Escherichia coli,in order to improve the detection rate of positive strains.Method：The local diarrhea syndromic surveillance hospital unused,watery,sticky pus or purulent blood sample diluted by those bacteria,a variety of selective culture medium screening pathogen antibiotics in patients with diarrhea from 2012 to 2013 ,after biochemical test, multiple PCR test and serotype tests were identified.Result：65 strains serum agglutination of diarrheogenic Escherichia coli were detected and belong to EPEC,EIEC,ETEC,the EPEC accounted for 83.08%,a total of 8 serotypes,serotype O55：H59,O128：H67,which accounted for 58.47%;there were 4 kinds of virulence related genes,among them 49 were escV（75.38%）. Serum without typing were 27 strains,5 species of virulence related genes were detected,which escV had 15 strain（55.56%）,belong to EPEC,EIEC,ETEC,EAEC.Conclusion：Local serological diarrhea syndrome is caused by Escherichia coli EPEC,common serotypes are O55：H59,O128：H67;virulence genes are escV,escV＋bfpB, invE,elt. Serum without typing diarrheogenic Escherichia coli virulence genes are escV,escV＋bfpB,invE,elt,astA, and consistent with typing of strains carrying genes. Detection of Escherichia coli diarrhea patients,basal medium,in bacterial isolation and conventional biochemical tests,serological identification,virulence gene detection is necessary to Escherichia coli,in order to improve the positive rate,to avoid detection,improve the diagnosis of diarrhea genic Escherichia coli.

关 键 词：致泻大肠埃希菌 血清型 毒力基因 

分 类 号：R440[医药卫生—诊断学]