细胞因子IL-1β和TNF-αmRNA在人精子表达的变化及意义  

The expression variations of IL-1 beta and TNF-alpha mRNA in human spermatozoa

作　　者：白文俊[1] 朱积川[1] 闫征[2] 邓庆平[1] 何培英[2] 

机构地区：[1]北京大学人民医院泌尿外科,100044 [2]北京大学人民医院,中心实验室100044

出　　处：《中华泌尿外科杂志》2001年第3期172-175,共4页Chinese Journal of Urology

摘　　要：目的　了解IL 1β和TNF α在人精子表达的变化及意义。 　方法　应用逆转录聚合酶链式反应 (RT PCR)检测 13例正常生育者、30例不育者及 30例慢性前列腺炎患者精子IL 1β和TNF αmRNA表达。　结果　不育者精子中IL 1β和TNF αmRNA表达均显著低于正常生育者 (P=0 .0 18和P =0 .0 46 )。慢性前列腺炎患者精子中TNF αmRNA的表达显著低于正常生育者 (P =0 .0 18) ,而IL 1βmRNA表达与正常生育者相比差别无显著性意义 (P =0 .42 )。IL 1β和TNF αmR NA在不育者与慢性前列腺炎患者精子中的表达差别无显著性意义 (P =0 .49和P =0 .6 2 )。　结论　某些因素可能干扰不育症患者精子IL 1β和TNF α的合成 ,导致精液质量下降及不育。生殖道炎症也可能影响精子TNF α表达 。Objective To investigate the expression variations of IL 1 beta and TNF alpha in human spermatozoa. Methods IL 1β and TNF α mRNA expressions were studied by means of reverse transcription polymerase reaction (RT PCR)in 13 fertile men,in 30 infertiles and in 30 chronic prostatitis patients. Results The expressions of IL 1β and TNF α mRNA in infertile men were significantly lower than those in fertile men( P =0.018 and P =0.050),and the expressions TNF α mRNA in chronic prostatitis patients were significantly lower than those in fertile men( P =0.018 ),but the expressions of IL 1β in these patients were not significantly lower than in fertile men( P =0.420). The expression differences of IL 1β and TNF α mRNA between the infertile men and chronic prostatitis patients were not significantly( P =0.488 and P =0.616). Conclusions Some unknown factors might impair the production of IL 1β and TNF α in infertile spermatozoa, and that may be responsible for the poor quality of semen and the infertile status in these patients. Furthermore, urogenital inflammation could also disturb the expression of TNF α in human spermatozoa.

关 键 词：精子 细胞因子 信使核糖核酸 IL-1β TNFΑ mRNA 生殖道感染 不育症 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]