长波紫外线照射对线粒体DNA缺失细胞系细胞自噬的影响研究  

The research on influence of autophagy in mtDNA-depleted cell lines with UVA irradiation

作　　者：范秀针[1] 陈胡林[2] 赵敏玲[2] 朱英杰[2] 高睿迪 杨慧兰[2] 刘仲荣[2] 

机构地区：[1]南方医科大学第一临床学院,广东广州510010 [2]中国人民解放军广州军区广州总医院皮肤科,广东广州510010

出　　处：《临床皮肤科杂志》2014年第7期397-401,共5页Journal of Clinical Dermatology

基　　金：国家自然科学基金(30972652)资助项目;广东省自然科学基金(915100100200020)资助项目

摘　　要：目的:探讨不同剂量长波紫外线(UVA)照射对线粒体DNA缺失细胞系细胞自噬的影响。方法:通过不同剂量(0、4、8、12 J/cm^2)长波紫外线(UVA)连续3 d对来自骨肉瘤细胞的两种细胞系ρ°206细胞(无线粒体DNA)和143B·TK-细胞(含线粒体DNA)照射诱导细胞发生自噬。通过电镜检测自噬小体;吖啶橙染色后荧光显微镜观察细胞自噬小体;以及吖啶橙染色后流式细胞仪测定发生自噬的细胞阳性率。结果:各组细胞均出现了自噬小体。143B·TK-细胞各剂量组细胞自噬体阳性细胞数百分率分别为(0.411 4±0.012 7)%,(0.625 2±0.018 5)%,(0.723 0±0.104 1)%和(0.766 1±0.012 3)%。143B·TK-细胞不同剂量组间两两比较差异均存在统计学意义(P<0.05);ρ°206细胞各剂量组(0、4、8、12.J/cm^2)自噬体阳性细胞数百分率分别为(0.367 3±0.0018)%,(0.1311±0.008 8)%,(0.327 3±0.010 9)%,(0.510 2±0.009 4)%。ρ°206细胞不同剂量组间两两比较差异均存在统计学意义(P<0.05)。143B·TK-细胞与ρ°206细胞同一剂量组之间发生自噬的阳性细胞率差异有统计学意义(P<0.05)。结论:UVA照射可诱导细胞自噬,其自噬率在一定范围内与剂量呈正相关,线粒体DNA缺失可促进UVA照射诱导的细胞自噬。Objective： To explorer the impact of antophagy in mtDNA-depleted cells by different doses of UVA irradiation. The impact of mitoehondrial DNA on the autophagy were detected after different doses of ultraviolet （UVA） irradiation. Methods： Autophagy of mtDNA depletion cells and wild-type steosarcoma were induced by different doses （0,4,8,12 J/cm2） of UVA irradiation for sequential 3 days. Autophagy was observed under TEM and under fluorescence microscope by acridine orange. The positive rate of autophagy were assayed throngh flow eytometry by acridine orange stained. Results： Outcome autophagic bodies were found among all groups. The autophagy positive percentage in different dose groups of 143B.TK- were （0.411 4±0.0127）%, （0.625 2±0.018 5）%, （0.723 0±0.104 1）% and （0.766 1±0.012 3）%. Each two group compared, the differences were statistically significant （P〈0.05）. In the same way, the autophagy posititive percentage of ρ°206 cells among different doses groups were（0.367 3±0.001 8）%, （0.131 1±0.008 8）%, （0.327 3±0.010 9）% and （0.510 2＋0.0094）%. The diference of the autophagy positive percentage among different groups were significantly（P〈0.05）. Conclusion： Autophagy is induced by UVA irradiation. Autophagy rate is depend on irradiation doses at a certain range. Autophagy is promoted in mtDNA depletion cells by UVA irradiation.

关 键 词：自噬 细胞 长波紫外线 线粒体DNA缺失 

分 类 号：R758.2[医药卫生—皮肤病学与性病学]