糖尿病牙周病大鼠牙周龈沟液中TNF-α,IL-1β和LPS水平的研究  

Study of TNF-α,IL-1β and LPS Levels in the Gingival Crevicular Fluid of a Rat Model of Diabetes Mellitus and Periodontitis

作　　者：姜竹玲[1] 高睿[2] 陈萌萌 李莹[4] 王祥[4] 张斌[1] 

机构地区：[1]哈尔滨医科大学附属第二医院口腔种植科,黑龙江哈尔滨150086 [2]哈尔滨医科大学附属口腔医学院正畸科 [3]哈尔滨市第四医院口腔科 [4]哈尔滨医科大学附属二院硬组织发育与矿化研究所

出　　处：《口腔医学研究》2014年第6期493-496,共4页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:81170960);教育部博士点基金(编号:20102307110006)

摘　　要：目的:本文主要研究了糖尿病牙周病大鼠模型龈沟液中TNF-α,IL-1β和LPS水平的改变。方法:15只大鼠通过注射链脲佐菌素建成糖尿病模型并使用丝线诱导成牙周病模型(组1),15只大鼠丝线诱导成牙周病模型(组2),15只大鼠链脲佐菌素注射建成糖尿病模型(组3),15只系统健康大鼠作为正常对照组(组4)。用ELISA方法检测龈沟液中TNF-α,IL-1β和LPS水平。结果:组1与组2、3、4相比TNF-α,IL-1β和LPS水平较高(P<0.05),组2中的水平比组3、4高(P<0.05),组3的比组4的水平高(P<0.05)。组间数据有统计学意义。结论:数据表明糖尿病可以提高牙周龈沟液中TNF-α,IL-1β和LPS的水平。Objective.. To investigate the dynamic changes in the levels of TNF-α, IL-1β and LPS in the GCF in a rat model of diabetes mellitus （DM） and periodontitis （PD）. Methods： DM and PD were induced together in 15 rats （group 1） by streptozotocin injection and ligature induction. Periodontitis alone was produced by ligature induction in 15 rats （group 2）, diabetes alone was produced by streptozotocin injection in 15 rats （group 3）, and fifteen systemically and periodontally healthy rats were used as controls （group 4）. The gingival TNF-α, IL-1β and LPS levels were measured by using ELISA method. Results： Higher levels of TNF-α, IL-1β and LPS were found in group 1 compared with group 2, group 3 and group 4 （P〈0.05）. The quantities of TNF-α, IL-1β and LPS were greater in group 2 than group 3 and group 4 （P〈0.05）. Group 3 demonstrated higher levels of TNF-α, IL-1β and LPS than group 4 （P〈0.05）. Statistically significant differences were noted between all groups. Conclusion： These data indicate that DM may lead to enhanced TNF-α, IL-1β and LPS production in the periodontal tissues.

关 键 词：肿瘤坏死因子-α 白介素-1Β 脂多糖 糖尿病 牙周病 

分 类 号：R781.4[医药卫生—口腔医学]