心脏移植患者3种牙周可疑致病菌的检测  

Detection of Periodontal Pathogens in Patients with Heart Transplantion

作　　者：曹莹[1] 吕亚林[1] 贾一新[2] 韩笑[1] 刘冬宇[1] 丁芳[1] 

机构地区：[1]首都医科大学附属北京安贞医院口腔医疗中心,北京100029 [2]首都医科大学附属北京安贞医院心外科

出　　处：《口腔医学研究》2014年第6期519-522,527,共5页Journal of Oral Science Research

基　　金：国家自然科学基金资助项目(编号:81170282);首都医科大学附属北京安贞医院院长科技发展基金资助项目(编号:2012Z06)

摘　　要：目的:应用聚合酶链反应法对心脏移植患者龈上菌斑中牙龈卟啉单胞菌、福赛坦氏菌和齿垢密螺旋体进行检测,分析其存在状况与牙周临床指标的关系。方法:55例心脏移植患者为实验组,36例正常成人为对照组。以16、26为受检牙位,分别采集龈上菌斑,应用PCR技术检测菌斑中的Pg,Tf,Td。同时记录受检牙位的菌斑指数、龈沟出血指数,龈沟探诊深度及附着丧失。结果:心脏移植组PLI、SBI、PD均高于对照组(P<0.05),心脏移植组龈上菌斑中Pg、Tf、Td检出率分别为为81.8%、69.1%、76.4%,对照组中3种细菌检出率分别为58.3%、41.7%、41.7%。检出率均高于对照组(P<0.05)。心脏移植组3种致病菌同时检测出率为53.6%(59/110),对照组3种致病菌同时检出率为29.2%(21/72),两组差异有统计学意义(P<0.05)。心脏移植组中3种致病菌均为阳性患者的PLI、SBI、PD均高于对照组(P<0.05)。结论:心脏移植患者口腔卫生状况较差,为防止牙周疾病的发生,应及早进行口腔卫生干预治疗。Objective： To detect the presence of Porphyromonas gingivalis（Pg）, Tannerellaforsythus（Tf）and Trepinema denticola （Td）in supragingival plaque from patients with heart transplantion using PCR, and investigate the relationship between the periodontal pathogens and clinical parameters. Methods： Supragingival plaque were collected from 55 patients with heart transplantion and 36 subjects without periodontitis. The samples were collected from the labial surfaces of both moxillary first molar. Extracted DNA from plaque samples was used for PCR analysis. Intraoral examination, plaque index （PLI）, sulcus bleeding index （SBI）, probing depth （PD） and attachment loss （AL） were performed and recorded. Results： PLI, SBI, and PD were higher in the patients with heart transplantion than the control group （P〈Z0.05）. There was no statistically difference in AL between two groups. Pg, Tf, Td were more frequently detected in the patients with heart transplantion （81.8% ,69. 1% ,76.4%） than that in the control group（58.3% ,41.7% ,41.7%） （P〈0.05）. The combined detection frequency of red complex （Pg-/-Tf-b Td） was 53.6% in the patients with heart transplantion （59/110）, significantly higher than the control group（29. 2 %, 21/72）. PLI, SBI, and PD of the heart transplantion patients with 3 putative periodontal pathogens were higher than the control group（P〈0.05）. Conclusion： Detection rates of putative periodontal pathogens including Pg, Tf, Td were high in the patients with heart transplantion. It is necessary to strengthen oral health education and disease control to the patients with heart transplantion.

关 键 词：心移植 龈上菌斑 聚合酶链式反应 

分 类 号：R781.4[医药卫生—口腔医学]