疱疹类病毒与喉良性及恶性上皮病变的关系  

Herpesviridae and laryngeal neoplasia

作　　者：林昶[1] 姜泗长[2] 杨伟炎[2] 韩东一[2] 易自翔[1] 

机构地区：[1]福建医科大学附属第一医院耳鼻咽喉科,福州350005 [2]解放军总医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉科杂志》2001年第3期122-124,共3页Journal of Clinical Otorhinolaryngology

摘　　要：目的 :探讨疱疹类病毒在喉良恶性上皮病变中的作用。方法 :采用通用引物多聚酶链反应 (PCR)及原位多聚酶链反应 (PCR- ISH)对 12 8例喉良恶性病变进行了检测。结果 :PCR检测 12 8例标本 ,10例检出单纯庖疹病毒 1型 (HSV - 1) ,其中喉癌 3例 ,原位癌 1例 ,息肉 4例 ,角化症 2例 ,未检出爱泼斯坦病毒 (EBV)及巨细胞病毒 (CMV )。 10例阳性标本用 PCR- ISH定位检测 ,除 1例鳞癌和 1例原位癌阴性外 ,其余 8例均阳性 ,良性病变从基底层到浅层细胞核均阳性 ,癌症中则散在阳性 ,1例腺体细胞核也呈阳性。结论 :EBV及 CMV与喉病变无关 ;大部分喉良恶性上皮病变与 HSV- 1无关 ,但在少数患者 ,HSV - 1可能起诱发作用。Objective:To investigate the relationship between herpesviridae and malignant or benign laryngeal diseases.Method:128 paraffin embedded laryngeal squamous cell carcinoma and laryngeal epithelium hyperplastic lesions were detected by polymerase chain reaction (PCR) and PCR ISH for herpesviridae. Result:HSV 1 was detected in 10 cases by PCR,among them 3 were laryngeal squamous cell carcinoma (LSCC),1 was carcinoma in situ(CIS),4 were laryngeal polyps and 2 were laryngeal keratosis. Except 1 LSCC and 1 CIS, 8 of 10 cases were positive while detected by PCR ISH. In benign diseases, signals were shown from basal layer to superficial cell; in malignant lesions, the signals were scattered in the diseases.Conclusion:Most of laryngeal diseases were not related to herpesviridae, but HSV 1 may acts as initiator in the development of a few cases.

关 键 词：喉肿瘤 疱疹病毒 多聚酶链反应 

分 类 号：R739.65[医药卫生—肿瘤]