Clusterin对膀胱癌细胞抗凋亡作用机制的研究  

Study of the antiapoptosis mechanism of clusterin in EJ bladder carcinoma cell line

作　　者：梁丽莉[1] 张强[1] 李钟[2] 王建中[3] 丁义[1] 张志文[1] 郭应禄[1] 

机构地区：[1]北京大学泌尿外科研究所,北京大学第一医院泌尿外科100034 [2]美国芝加哥西北大学泌尿分子生物学系 [3]北京大学第一医院检验科

出　　处：《中华泌尿外科杂志》2001年第1期31-33,共3页Chinese Journal of Urology

摘　　要：目的　探讨新的抗细胞凋亡因子Clusterin对膀胱癌EJ细胞的作用机制。　方法　用丝裂霉素 (MMC)诱导膀胱癌EJ细胞凋亡 ,采用流式细胞术 (FCM)检测不同浓度的Clusterin对抗EJ细胞的凋亡率。　结果　小剂量Clusterin可以特异性抑制MMC诱导的EJ细胞凋亡作用。　结论Clusterin通过抗凋亡机制作用于膀胱癌EJ细胞 ,可能与膀胱癌细胞的复发、耐药等临床特性有关 ,此项研究对阐明膀胱癌发生发展的生物学机制以及临床应用Clusterin抗体治疗膀胱癌提供帮助。Objective To study the mechanism of novel antiapoptosis factor clusterin in EJ cell. Methods MMC was used to induce apoptosis of EJ cells and the ratio of apoptosis was studied by flowcytometry after the use of clusterin. Results Clusterin in low dose can inhibit the apoptosis induced by MMC. Conclusions Clusterin can influence the development of bladder cancer by antiapoptosis.The expression of clusterin may have some relationship with the drug resistance or recurrence of bladder cancer.The study of clusterin is helpful to elucidate the biological mechanism of the bladder cancer and direct the therapy of bladder cancer using the antibody of clusterin.

关 键 词：CLUSTERIN 膀胱肿瘤 癌 凋亡 

分 类 号：R737.14[医药卫生—肿瘤]