大鼠脑挫伤后非损伤侧脑组织的ADC 值及病理改变  

The variation of ADC values and pathological change in non-injured side tissues of traumatic brain injury of rat

作　　者：雷小燕[1] 鲁宏[1] 

机构地区：[1]中南大学湘雅医学院附属海口医院放射科,海南海口570208

出　　处：《实用放射学杂志》2014年第6期1026-1029,1034,共5页Journal of Practical Radiology

基　　金：国家自然科学基金资助项目（81160181）;海南省国际合作项目[琼科（2012）65号].

摘　　要：目的：探讨大鼠脑挫伤（TBI）后非损伤侧脑组织表观扩散系数（ADC ）变化及其病理改变。方法选健康成年 Wistar 大鼠60只,制作中度脑挫伤模型,随机分为对照组及创伤组（1 h、3 h、6 h、12 h 及24 h 组）,每组各10只,各时相点分别进行头部 MR-DWI 检查,测量损伤侧与镜像非损伤侧区的 ADC 值,取脑进行组织学观察、IgG 免疫组化染色半定量观察血脑屏障（BBB）的通透性,所得值进行统计学分析。结果对照组双侧脑组织结构及 DWI 未见异常,IgG 染色阴性。创伤组非损伤侧1 h 脑组织结构及DWI 未见异常,IgG 染色阴性。3 h 出现细胞内水肿,IgG 染色弱阳性。6 h 细胞内水肿加重,同时出现血管源性水肿,IgG 染色呈强阳性。12~24 h 细胞内水肿仍继续加重,而血管源性水肿有缓解趋势,IgG 染色减弱,并见小胶质细胞增生。非损伤侧 ADC 值与对照组比较差异无统计学意义（P 〉0.05）。损伤侧脑组织在1 h 即出现血管源性水肿,3 h 出现细胞内水肿,随时间2种水肿逐渐加重。ADC 值在1 h 升高、然后开始降低至6 h,6 h 后开始回升直至24 h,呈现“V”形变化趋势。结论当一侧脑挫伤时,非损伤侧的脑组织有相应的病理变化,在时间上滞后于损伤侧,首先出现的是细胞内水肿,然后发生血管源性水肿,随损伤时间延长血管源性水肿有缓解趋势,但 ADC 值无显著变化,呈现“假性正常”现象。Objective This study was designed to investigate the correlation between the apparent diffusion coefficient（ADC）value and pathological change of the non-injured side brain tissue of traumatic brain injury（TBI）of rat.Methods Sixty healthy adult Wistar rats were randomly divided into two groups：the control or TBI groups.The TBI group was divided into five sub-groups according to the different time intervals：1,3,6,12,24 h（n = 10）.The animal brain of each group was scanned with MR-DWI,and the ADC value of damaged areas and contralateral non-damaged areas were measured.After that the brains were taken out at different time points after TBI.The cerebral edema and blood-brain barrier（BBB）changes in structure were examined with an optical microscopy and transmission electron microscopy,and the IgG content in the same tissues were determined by means of immunohistochemistry.The data was analyzed with SPSS 13.0 statistical software.Results There was no signal abnormality on MR-DWI and tissue structure in control group,and the IgG stain was negative.In the contralateral non-damaged areas of TBI group,pathological observation revealed no variation at 1 h after TBI,and the IgG stain was also negative.Cellular edema was shown at 3 h and the IgG stain was slight positive.The cellular edema aggravated with time and angioedema appeared at 6 h.IgG stain was shown significantly posi-tive.At 12~24 h,cellular edema kept increasing more severe,however angioedema had a trend to mitigated along with time,IgG stain became slight and the proliferation of glial cells could observed.Compared with control group,the ADC values of the contralateral non-damaged areas in each group showed no significant difference （P 〉0.05）.However,in the damaged areas of each group, angioedema appeared at 1 h and gradually aggravated,cellular edema occurred at 3 h,Both of them were aggravated with time. ADC values increased at 1 h,and then decreased until 6 h,then followed a climbing up to 24 h,showed as a “V”-shaped.Con-clusion Wh

关 键 词：脑挫伤 非损伤侧 病理 血脑屏障 IGG 扩散加权成像 

分 类 号：R742[医药卫生—神经病学与精神病学] R446.8[医药卫生—临床医学]