脑出血对大鼠前脑侧脑室室管膜下区细胞的影响及三七总皂苷的作用  

Effect of cerebral haemorrhage and panax notoginseng saponins on subventricular zone cells of lateral ventricle in rat forebrain

作　　者：白华静 刘峻崎 李巾伟[1] 司银楚[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《中华中医药杂志》2014年第6期1801-1804,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金面上课题(No.81373830)~~

摘　　要：目的:观察脑出血对大鼠前脑侧脑室室管膜下区(SVZ)细胞的影响及三七总皂苷的作用。方法:采用Wistar成年雄性大鼠,用胶原酶注入尾壳核建立脑出血模型,应用免疫组化方法观察脑出血后侧脑室SVZ细胞增殖细胞核抗原(PCNA)、β-Ⅲ型微管蛋白(Tuj-1)、胶质原纤维酸性蛋白(GFAP)的表达,以及碱性成纤维细胞生长因子(bFGF)的表达与活化,研究侧脑室SVZ细胞的增殖、分化及三七总皂苷的作用。结果:通过连续切片的免疫组化结果显示脑出血后损伤侧侧脑室SVZ的PCNA、GFAP、Tuj-1和bFGF阳性细胞表达增强,给予三七总皂苷干预后,给药组上述阳性细胞个数显著增多,与模型组比较具有显著性差异(P<0.05,P<0.01)。结论:脑出血能激活并促进侧脑室SVZ细胞增殖和分化,三七总皂苷可能通过增强前脑bFGF的表达而促进SVZ细胞增殖和分化。Objective： To observe effect of cerebral haemorrhage and panax notoginseng saponins （PNS） on subventricular zone （SVZ） cells of lateral ventricle in rat forebrain. Methods： Cerebral hemorrhage model were established by injecting with collagenase on caudate nucleus of adult male Wistar rats. The expression of proliferating cell nuclear antigen （PCNA）, Tuj-1, glial fibrillary acidic protein （GFAP） and the expression and activation of basic fibroblast growth factor （BFGF） were determined by immunohistochemical method on SVZ cells of lateral ventricle after cerebral haemorrhage. To study the proliferation and differentiation of SVZ cells of lateral ventricle and the effect of PNS. Results： Through the immunohistochemical results of serial sections, the expression of PCNA, GFAP, Tuj-1 and bFGF positive cells increased in SVZ of lateral ventricle after cerebral haemorrhage. After the administration of PNS, the numbers of positive cells in the treatment group increased significantly, there were significant differences compared with model group （P〈0.05, P〈0.01）. Conclusion： Cerebral hemorrhage could activate neural stem cells in SVZ of lateral ventricle in rats forebrain and promote proliferation and differentiation. PNS could promote the above effects through enhancing the forebrain BFGF expression.

关 键 词：脑出血 室管膜下细胞 三七总皂苷 增殖 分化 

分 类 号：R743.34[医药卫生—神经病学与精神病学]