四逆散对蛋氨酸-胆碱缺乏饮食诱导的小鼠非酒精性脂肪肝炎的研究  

Effect of Sini Powder on non-alcoholic fatty hepatocirrhosis induced by methionine-choline deficit diet

作　　者：罗闳丹[1] 林丽美[1] 庹勤慧[1] 姚海伦[2] 孙少卫[1] 廖端芳[1] 

机构地区：[1]湖南中医药大学药学院,湖南长沙410001 [2]南华大学,湖南衡阳421001

出　　处：《药物评价研究》2014年第3期231-234,共4页Drug Evaluation Research

摘　　要：目的研究四逆散对蛋氨酸-胆碱缺乏(methionine-choline-deficient,MCD)饮食诱导的小鼠非酒精性脂肪肝炎的作用及机制。方法通过MCD饮食诱导小鼠非酒精性脂肪肝炎模型,并同时预防性给药普罗布考及四逆散4周,HE染色观察各组小鼠肝组织病理改变;检测小鼠血清丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、三酰甘油(TG)、含量以及肝脏组织TG、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活力。结果四逆散高剂量组小鼠血清ALT、AST均有下降,血清中TG含量升高,且肝脏TG含量降低;病理观察显示四逆散高剂量组小鼠肝细胞内脂肪空泡减少,炎性细胞浸润减少;同时肝组织SOD活力增加,MDA含量降低。结论高剂量四逆散对MCD饮食诱导的小鼠非酒精性脂肪肝炎有预防作用,其主要通过调节血清和肝脏TG的分布,增加肝组织抗氧化能力,减少体内过氧化物损害来实现。Objective To study the effect of Sini Powder on non-alcoholic fatty hepatocirrhosis（NASH） induced by methionine- choline deficit（MCD） diet. Methods The model mice of NASH were administered with probucol and Sini Powder for 4 weeks. Then the pathological changes of liver were observed by HE staining, the contents of ALT, AST, TG, and MDA and the activity of SOD were tested and compared among groups. Results Compared with the model group, the ALT and AST contents in serum were lower, TG content in serum was reduced, and TG content in liver was higher in high-dose Sini Powder group. The pathological observation showed that the lipo-vacuoles were reduced in hepatocytes, and the infiltration of inflammatory cell was decreased. The activity of SOD was increased while the content of MDA was decreased. Conclusion High-dose Sini Powder can prevent the course of NASH induced by MDC diet by adjusting TG distribution in serum and liver, increasing the anti-oxidative capacity of liver, and reducing the peroxide damage.

关 键 词：四逆散 非酒精性脂肪肝炎 MCD饮食 氧化应激 

分 类 号：R575.5[医药卫生—消化系统]