一株甲基对硫磷降解菌-布朗克假丝酵母JMUPMD-1的分离与鉴定  

Isolation and Identification of a Parathion-methyl Degrading Fungus-Candida blankii JMUPMD-1

作　　者：张霞[1] 张书泰 谢顺昌 杜希萍[1,2,3] 李利君[1,2,3] 

机构地区：[1]集美大学生物工程学院,福建厦门361021 [2]福建省食品微生物与酶工程重点实验室,福建厦门361021 [3]福建省高校食品微生物与酶工程技术研究中心,福建厦门361021

出　　处：《激光生物学报》2014年第2期183-188,共6页Acta Laser Biology Sinica

基　　金：福建省烟草公司科技项目(2012049)

摘　　要：对一株新分离的、能以甲基对硫磷为唯一磷源生长的菌株JMUPMD-1,利用形态特征观察和生理生化特性,及结合rDNA ITS分子序列分析对JMUPMD-1进行鉴定;采用气相色谱法检测培养过程中甲基对硫磷浓度的变化,确定甲基对硫磷降解速率。该菌株的rDNA ITS序列与布朗克假丝酵母(Candida blankii)的同源性为99%,形态特征和生理生化特性与布朗克假丝酵母相符合,因此鉴定为布朗克假丝酵母。以350μg/L甲基对硫磷为唯一磷源和350μg/L甲基对硫磷及1 g/L K2HPO4组成的混合磷源培养该菌株,测得该菌株的降解率为48.6%。该菌株的胞内提取液具有明显的甲基对硫磷降解酶活性。A newly isolated fungus JMUPMD-1, capable of growing with parathion-methyl as the sole phosphorus source, was identified based on its physiological and biochemical property, morphological characteristics and rDNA ITS sequences analysis, and its capability of degrading parathion-methyl was investigated by determining the changes of para thion-methyl concentration in the broth of the isolate. JMUPMD-1 ＇ s colonic and microscopic morphologies were identical with and its sequences of rDNA ITS was 99% similar to Candida blankii. Consequently, it was identified as Candida blankii. When cultured with an artificial medium containing parathion-methyl as the sole phosphorus or parathion-methyl- K2HPO4 mixed phosphorus, the degradation rate of parathion-methyl was 48.6%. JMUPMD-1 ＇ s intracellular extract had obvious parathion-methyl degrading enzyme activity.

关 键 词：甲基对硫磷 生物降解 ITS序列 布朗克假丝酵母 菌种鉴定 

分 类 号：Q933[生物学—微生物学]