生育和不育男性精液维生素B_(12)、叶酸、活性氧和精子质量的比较  

Comparison of seminal vitamin B_(12), folate, reactive oxygen species and various sperm parameters between fertile and infertile males

作　　者：陈起萱[1] VNg 梅节[1] SEChia SKTay 凌文华[1] CNOng 

机构地区：[1]中山医科大学公共卫生学院,广州510089 [2]Department of Community,Occupational&Family Medicine,the National University of Singapore [3]Department of Obstetrics&Gynaecology,Singapore General Hospital

出　　处：《卫生研究》2001年第2期80-82,共3页Journal of Hygiene Research

基　　金：美国中华医学基金会 ( CMB)资助项目!( No.EOH98)

摘　　要：为了探讨精液维生素 B1 2 (VB1 2 )、叶酸和活性氧 (ROS)对精子质量和生殖功能的影响。收集成年男性不育者 44名 (不育组 )和经证实配偶已怀孕的健康成年男性 176名 (生育组 )的精液 ,根据 WHO推荐方法检测精子质量指标 ,并测定精液 VB1 2 、叶酸和 ROS水平。结果显示二组 VB1 2 和叶酸没有显著性差异 (P>0 .0 5 ) ,不育组 ROS和形态异常精子都显著高于生育组 (P<0 .0 1) ,ROS与精子形态异常呈正相关 (P<0 .0 1) ,VB1 2 和叶酸与 ROS均呈负相关 (P<0 .0 1)。结论认为精液 ROS升高 ,可能促使精子形态异常 ,从而影响男性生殖功能。Vitamin B 12 (VB 12 ), folate and reactive oxygen species(ROS)in human semen from both fertile and infertile males,and their relationships with other parameters were observed. Semen samples from 44 infertile and 176 fentile control subjects were collected and measured based on the guidelines issued by WHO.The results showed that no significant differences on semen VB 12 and folate were observed between fertile and infertile groups( P >0 05).However,the levels of ROS in infertile group were significantly higher than those in fertile group( P <0 01).The morphological defects and low motility of sperm in infertile group was significantly higher than those in fertile group( P <0 01).A positive relation was observed between the levels of ROS production and the morphological defect of sperm( P <0 01).There was a significant negative correlation between the levels of VB 12 ,folate and ROS( P <0 01) in semen.It is interesting to note that the values of ROS also show a significantly positive correlation with sperm motility (P <0 01).A significant differences of ROS levels between fertile and infertile group with negative white blood cells in sperm was alsoobserved.It is concluded that the inerease of ROS on the morphological defect of sperm is one of the most important factors related to poor sperm quality and human infertility.

关 键 词：维生素R12 叶酸 活性氧 精子质量 男性不育症 精液 

分 类 号：R698.2[医药卫生—泌尿科学]