胞必佳诱导人肝癌细胞HepG_2凋亡的研究  

Study on the Induction of N-CWS to the Apoptosis in Human Hepatocellular Carcinoma

作　　者：雷艳[1] 林锦彬[1] 谢必峰[1] 

机构地区：[1]福建师范大学生命科学学院,福建福州350108

出　　处：《药物生物技术》2014年第1期57-60,共4页Pharmaceutical Biotechnology

摘　　要：为研究注射用胞必佳在体外诱导人肝癌细胞HepG2凋亡的作用与机制,采用MTT法和LDH法检测胞必佳对HepG2细胞的增殖抑制作用、细胞毒性作用;AO/EB双荧光染色观察细胞形态学变化;流式细胞术检测细胞周期分布。结果显示,胞必佳作用HepG2细胞后,呈浓度依赖性抑制HepG2细胞的生长,作用24,48,72 h IC50分别为:187.53,155.31,92.54μg/mL;胞必佳对HepG2细胞24,48 h的致死率达68%左右。AO/EB荧光染色可见细胞出现明显的凋亡形态学改变。不同浓度胞必佳对HepG2细胞周期的影响表现为G1期阻滞,G2/M期和S期细胞所占比例减小。胞必佳对HepG2细胞有明显的生长抑制作用,并诱导肿瘤细胞凋亡,其抗肿瘤作用的机理可能是:通过干扰细胞周期调控点,主要阻止G1期细胞向G2/M期的转变。In order to study the mechanism of Nocardia rubra cell wall skeleton N-CWS in vitro induced apoptosis of human hepatocellular carcinoma cell line HepG2,MTT and LDH methods were used to determine the effects of Nocardia rubra cell wall skeleton NCWS on human hepatocellular carcinoma cell line HepG2,which could inhibit the proliferation and induce the apoptosis.The morphological change of the cells with AO/EB double fluorescent staining was also abserved.Lastly,Flow cytometry was used to detect the cell apoptosis and cell cycle.The results showed that compared with the control group,the effect of N-CWS on the cells,in a concentration-dependent manner inhibited the growth of HepG2cells.After being inhabited 24,48,72 h,the IC50value was 187.53,155.31 and 92.54 μg/mL respectively.The death rate average was of 68%,while N-CWS on HepG2cell was 24,48 h.Through AO/EB double fluorescent staining,the obviously morphologic changes of apoptosis visible cells were found.Different concentrations of N-CWS showed different effects on cell cycle and it decreased cell percen-tages in G2/M and S phases,but increased cell percentage in G1phase.The different concentrations of N-CWS on the apoptosis rate were 2.34%,21.43%,5.35% respectively.N-CWS can significantly inhibit the growth of HepG2cells,and induction of apoptosis in tumor cells.The mechanism of anti-tumor effect may be：To interference of cell cycle,the main block of cells in G1phase transition to the G2/M phase.

关 键 词：胞必佳 人肝癌HEPG2细胞 细胞凋亡 

分 类 号：R733[医药卫生—肿瘤]