电刺激大鼠上矢状窦区硬脑膜后血浆与脑组织中降钙素基因相关肽、P物质和前列腺素E_2释放差异  

Release difference of calcitonin gene-related peptide,substance P and prostaglandin E_2 in orbital venous plexus plasma and brain tissues from rats with dura mater of superior sagittal sinus following electrical stimulation

作　　者：陈健[1] 吕新勇[1] 孙晓萍[1] 陈春苗[1] 周军[1] 王振中[1] 萧伟[1] 

机构地区：[1]江苏康缘药业股份有限公司中药制药过程新技术国家重点实验室江苏省企业院士工作站,江苏连云港222001

出　　处：《中国药理学与毒理学杂志》2014年第3期329-333,共5页Chinese Journal of Pharmacology and Toxicology

摘　　要：目的探讨电刺激大鼠上矢状窦区硬脑膜制备偏头痛模型的可行性。方法雄性SD大鼠,脑部电极植入术后行电压3 V、频率6 Hz、脉宽0.25 ms、时间1 h电刺激,并于刺激0,15和30 min和1 h进行眼眶采血,采血后取脑组织。采用放射免疫法检测血浆和脑组织中降钙素基因相关肽(CGRP)、P物质和前列腺素E2(PGE2)含量。结果手术对照组与正常对照组大鼠脑组织中CGRP含量、P物质含量和PGE2含量为均无显著性差异;模型组大鼠脑组织中CGRP、P物质和PGE2分别为3.41±0.93,1.80±0.64和(5.17±0.69)ng·g-1,与手术对照组大鼠相比均有显著性差异(P<0.05)。各时间点手术对照组与模型组大鼠外周静脉血浆中CGRP、P物质和PGE2含量无显著性差异。结论电刺激大鼠上矢状窦区硬脑膜后,脑组织中CGRP、PGE2和P物质水平与外周静脉血中显著不同,表明可采用本方法制备偏头痛模型。OBJECTIVE To explore the feasibility of preparing a migraine model using rat dura mater of superior sagittal sinusin following electrical stimulation. METHODS Male SD rats were exposed to 1 h electrical stimulation after brain electrode implantation,at 3 V,6 Hz,and a pulse of 0. 25 ms. At 0,15,30 min,and 1 h,orbital blood and brain tissue were taken. The content of calcitonin gene-related peptide（ CGRP）,substance P and prostaglandin E2（ PGE2） in the plasma and brain tissue was detected by radio immunoassay. RESULTS The brain tissue content of CGRP,substance P and PGE2in the sham group and normal group was not significantly different. CGRP,substance P and PGE2in brain tissue of model group were 3. 41 ± 0. 93,1. 80 ± 0. 64,3. 41 ± 0. 93 and（ 1. 80 ± 0. 64） ng·g^-1 respectively,significantly different from sham group（ P〈0. 05）. At different time points,the content of CGRP,substance P and PGE2in plasma orbital venous plexus showed no significant difference between sham group and model group. CONCLUSION The release of CGRP,PGE2and substance P in brain tissue is significantly different from that of plasma after electrical stimulation. The results suggest that a model of migraine can be constructed by electrical stimulation.

关 键 词：偏头痛 电刺激 硬脑膜 降钙素基因相关肽 P物质 前列腺素E2 

分 类 号：R747.2[医药卫生—神经病学与精神病学]