Effect of Shengmai Injection(生脉注射液) on Diaphragmatic Contractility in Doxorubicin-Treated Rats  

作　　者：葛敏 方迎艳 刘国平 关宿东 

机构地区：[1]Department of Physiology,Bengbu Medical College

出　　处：《Chinese Journal of Integrative Medicine》2014年第1期43-48,共6页中国结合医学杂志（英文版）

摘　　要：Objective：To explore the diaphragmatic toxicity in doxorubicin（DOX）-treated rats and the related mechanisms,as well as the effects of Shengmai Injection（SMI,生脉注射液）on the diaphragmatic dysfunction.Methods：Thirty Sprague-Dawley male rats were randomly divided into three groups：control,DOX-treated and DOX＋SMI treated groups.DOX was given to rats in DOX and DOX＋SMI groups in 6 equal doses[2.5 mg/kg,intraperitoneal injection（i.p.）],on alternate days,over a period of 2 weeks for a cumulative dose of 15 mg/kg.SMI was given to DOX＋SMI rats in 12 doses（3 mL/kg,i.p.）for a period of 2 weeks before the administration of DOX and 2 weeks during the administration of DOX.The rats in the control group received equal volume of normal saline.Subsequently,the twitch and tetanic characteristics and force-frequency relationships,and the malondialdehyde（MDA）levels and the superoxide dismutase（SOD）activities,as well as the mRNA content and proteins of inducible nitric oxide synthase（iNOS）were determined.Results：The DOX-treated rats had decreased the peak twitch tension（Pt）,maximal tetanic tension（P0）and force-frequency relationship as compared with the control rats（P〈0.01）,while the diaphragm contractility in rats treated with SMI were significantly higher than that in DOX-treated rats（P〈0.01）.The DOX-treated rats had increased MAD levels and decreased SOD activities（P〈0.05）,and SMI decreased the MDA levels and increased the SOD activities in DOX-treated rats（P〈0.05）.Ultrastructure of diaphragm in the DOX-treated rats revealed typical alterations including fracture of diaphragm fibers,and edema and degeneration of mitochondria;these changes were relieved by SMI treatment.The mRNA content and protein of iNOS in DOX-treated rats were remarkably higher than those in control rats（P〈0.01）,while SMI decreased the mRNA expression level of iNOS in DOX-treated rats（P〈0.05）.Conclusions：Lipid peroxidation is responsible for DOX-induced diaphragm toxiObjective：To explore the diaphragmatic toxicity in doxorubicin（DOX）-treated rats and the related mechanisms,as well as the effects of Shengmai Injection（SMI,生脉注射液）on the diaphragmatic dysfunction.Methods：Thirty Sprague-Dawley male rats were randomly divided into three groups：control,DOX-treated and DOX＋SMI treated groups.DOX was given to rats in DOX and DOX＋SMI groups in 6 equal doses[2.5 mg/kg,intraperitoneal injection（i.p.）],on alternate days,over a period of 2 weeks for a cumulative dose of 15 mg/kg.SMI was given to DOX＋SMI rats in 12 doses（3 mL/kg,i.p.）for a period of 2 weeks before the administration of DOX and 2 weeks during the administration of DOX.The rats in the control group received equal volume of normal saline.Subsequently,the twitch and tetanic characteristics and force-frequency relationships,and the malondialdehyde（MDA）levels and the superoxide dismutase（SOD）activities,as well as the mRNA content and proteins of inducible nitric oxide synthase（iNOS）were determined.Results：The DOX-treated rats had decreased the peak twitch tension（Pt）,maximal tetanic tension（P0）and force-frequency relationship as compared with the control rats（P〈0.01）,while the diaphragm contractility in rats treated with SMI were significantly higher than that in DOX-treated rats（P〈0.01）.The DOX-treated rats had increased MAD levels and decreased SOD activities（P〈0.05）,and SMI decreased the MDA levels and increased the SOD activities in DOX-treated rats（P〈0.05）.Ultrastructure of diaphragm in the DOX-treated rats revealed typical alterations including fracture of diaphragm fibers,and edema and degeneration of mitochondria;these changes were relieved by SMI treatment.The mRNA content and protein of iNOS in DOX-treated rats were remarkably higher than those in control rats（P〈0.01）,while SMI decreased the mRNA expression level of iNOS in DOX-treated rats（P〈0.05）.Conclusions：Lipid peroxidation is responsible for DOX-induced diaphragm toxi

关 键 词：Shengmai Injection DOXORUBICIN DIAPHRAGM lipid peroxidation nitric oxide 

分 类 号：R285.5[医药卫生—中药学]