全反式维甲酸对人胃腺癌细胞株BGC-823迁移的影响  

Effect of all-trans retinoic acid on the migration of human gastric cancer BGC-823 cells

作　　者：杨艳艳[1] 胡安拉[1,2] 张素梅[1] 周青[1] 汪渊[1] 

机构地区：[1]安徽医科大学分子生物学实验室、生物化学与分子生物学教研室、安徽省/省部共建教育部重要遗传病基因资源利用重点实验室,合肥230032 [2]安徽医科大学公共卫生学院营养与食品卫生学系,合肥230032

出　　处：《安徽医科大学学报》2014年第7期867-871,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81272399);安徽省自然科学基金(编号:090413116)

摘　　要：目的研究全反式维甲酸(ATRA)对人胃腺癌细胞株BGC-823细胞迁移能力的影响及其可能机制。方法不同浓度ATRA处理BGC-823后,MTT法检测ATRA对细胞增殖的影响并计算半数抑制率(IC50),倒置显微镜下观察细胞形态学变化,划痕法检测其对细胞迁移能力的作用,平板克隆法观察其对细胞体外克隆形成能力的影响,Western blot法检测可能与迁移相关蛋白的表达。结果 ATRA对BGC-823细胞增殖具有明显的抑制作用,呈浓度依赖性,IC50为42.402 5μmol/L;ATRA可抑制细胞生长速度,使细胞形态拉长;ATRA可抑制BGC-823细胞的迁移;ATRA可明显降低细胞克隆形成数(P<0.05);Western blot结果显示ATRA显著降低肌球蛋白轻链激酶(MLCK)的表达和肌球蛋白轻链(MLC)的磷酸化。结论 ATRA对人胃腺癌细胞株BGC-823细胞的迁移具有抑制作用,可能与降低MLCK表达和MLC磷酸化水平有关。Objective To explore the effect of all-trans retinoic acid （ ATRA ） on the migration of human gastric cancer BGC-823 cells and its probable mechanism. Methods MTT assay was performed to measure the proliferation of BGC-823 cells treated with different concentrations of ATRA and calculated the IC50 . Light microscope was used to observe morphologic changes. The effect of ATRA on the migration of BGC-823 cells was analyzed by wound healing assay. The effect of ATRA on the colony formation rate of BGC-823 cells was measured by plate colony formation assay. The expression of proteins involved in migration was detected by Western blot. Results ATRA could inhibit the proliferation of cell line BGC-823 significantly. With the increase of concentration,the inhibition rate was more obvious and the IC50 was 42. 402 5 μmol/L. ATRA could inhibit cell growth and made them elongated. ATRA could decrease the distances of migration of BGC-823. ATRA could decrease the colony formation of BGC-823 significantly, compared with DMSO control（P〈0. 05）. ATRA could inhibit the expression of MLCK and phosphorylation of MLC protein which was detected by Western blot. Conclusion ATRA can inhibit the migration of human gastric carcinoma cell line BGC-823,which may be related to the down regulation of expression of MLCK and phosphorylation of MLC protein.

关 键 词：ATRA 胃癌 迁移 肌球蛋白轻链激酶 肌球蛋白轻链 

分 类 号：R735[医药卫生—肿瘤] R966[医药卫生—临床医学]