栀子苷衍生物五乙酰栀子酸对人肝细胞损伤的保护作用  

Protective effect of Gardenoside derivative on human hepatocyte injury

作　　者：程畅[1] 黄成[1] 王雅蕊 张磊[1] 汤文建[1] 李俊[1] 

机构地区：[1]安徽医科大学药学院,安徽医科大学肝病研究所,安徽天然药物活性研究省级实验室,合肥230032

出　　处：《安徽医科大学学报》2014年第7期946-949,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81072686、81273526)

摘　　要：目的探讨栀子苷衍生物五乙酰栀子酸对L-O2型肝细胞化学损伤的保护作用及其机制。方法培养L-O2型肝细胞,采用CCl4和H2O2体外诱导L-O2,检测培养上清液中谷草转氨酶(AST)、谷丙转氨酶(ALT)和乳酸脱氢酶(LDH)的水平,检测上清液中丙二醛(MDA)的含量、过氧化物歧化酶(SOD)的活力及谷胱甘肽过氧化物酶(GSH-Px)的活性。结果五乙酰栀子酸(10、50、100μg/ml)不仅可明显降低由CCl4所致肝细胞培养上清液中AST、ALT、LDH水平及MDA含量的升高,显著提高SOD的活力及GSH-Px的活性;而且还可使H2O2所致肝细胞培养上清液中升高的AST、ALT、LDH及MDA的含量明显降低,提高SOD的活力及GSH-Px的活性。结论栀子苷衍生物五乙酰栀子酸体外肝细胞损伤有显著的保护作用,其作用机制可能与其抗氧化作用有关。Objective To determine the protective effects of Gardenoside derivative on L-O2 induced by CCl4 or H2 O2 and to reveal the mechanism. Methods L-O2 hepatocyte cells were incubated and then injured by CCl4 or H2O2. The levels of serum alanine aminotransferase （ALT）,aspartate aminotransferase （AST） activity and liver homogenate superoxide dismutase （ SOD） ,malondialdehyde （ MDA） ,glutathione peroxidase （ GSH-Px） were measured. Results Not only the CCl4-induced L-O2 hepatocyte cells increased AST,ALT,LDH,MDA levels, and the loss of SOD and GSH-Px activities were remarkably attenuated by Gardenoside derivative（10,50,100 μg/ml）,but also the H2 O2-induced elevation of ALT, AST, LDH levels in the supernatant of hepatocytes increased MDA content of hepatocytes,and loss of SOD and GSH-Px activities were significantly attenuated by Gardenoside derivative （10,50,100 μg/ml）. In addition, gardenoside derivative had a better effect on the protective of human hepatocyte injury than gardenoside. Conclusion Gardenoside derivative is good at protecting hepatocytes than gardenoside from in vitro injury induced by CCl4 or H2 O2 and the mechanism might be related to the anti-oxidant process.

关 键 词：栀子苷 L-O2 型肝细胞 保肝 抗氧化作用 

分 类 号：R575.5[医药卫生—消化系统] R329.2[医药卫生—内科学]