Treg、CD8^+T细胞在食管胃交界部腺癌中的分布及其与临床病理参数的关系  

The expression of Foxp3 and CD8 in adenocarcinoma of esophagogastric junction(AEG) and their clinicopathological parameters meaning

作　　者：张舒平[1] 徐阿曼[1,2] 韩文秀[1] 王岳君[3] 汪礼坤[2] 黄雷[1] 

机构地区：[1]安徽医科大学第一附属医院普通外科一病区,合肥230022 [2]安徽医科大学第四附属医院普通外科,合肥230022 [3]安徽医科大学第四附属医院病理科,合肥230022

出　　处：《安徽医科大学学报》2014年第7期966-969,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省2012年度第三批科技计划(编号:12070403061)

摘　　要：目的探讨调节性T细胞(Treg)和CD8+T细胞在食管胃交界部腺癌(AEG)组织中的分布情况,分析两者与AEG的发生发展和临床病理资料的相关性。方法实验组:收集68例AEG癌组织病理蜡块;对照组:选用本院收治的20例胃溃疡患者的病理蜡块。应用免疫组织化学方法对实验组以及对照组的胃溃疡组织进行染色,以Foxp3作为Tregs的标记,检测其和CD8+T细胞的表达水平,并对两者在实验组中和对照组的分布水平与TNM分期、分化程度、淋巴转移、肿瘤的部位的关系等组别内进行分析。结果 1实验组中Foxp3、CD8+T细胞分布阳性率明显高于对照组,差异有统计学意义(P<0.05)。2实验组中有淋巴结转移组Foxp3+的分布阳性率高于无淋巴结转移组(P<0.05),肿瘤组织学分级为低分化组Foxp3+的分布阳性率显著高于组织学分级为高-中分化组(P<0.01),肿瘤的TNM分期较晚(Ⅲ)组要明显高于TNM分期较早(Ⅰ~Ⅱ)组(P=0.001)。3肿瘤组织中Foxp3和CD8在AEG中的分布呈负相关(r=-0.314,P=0.01)。结论 Foxp3+Treg的表达水平与AEG的发生发展密切相关,AEG肿瘤组织中Treg和CD8+T细胞的分布呈负相关,Treg可能通过抑制CD8+T细胞的功能,促进肿瘤的发展。Objective To explore the expression of regulatory T cell （ Treg ） and CD 8 ^＋ T cell in tissues of adenocarcinoma of esophagogastric junction （ AEG） , and analyze their correlation with carcinogenesis and tumor progres-sion. Methods A total of 68 AEG tissues were collected, with 20 peptic ulcer tissues selected as control. We dyed the tissues using immunohistochemistry, evaluating the expression of CD8 ^＋ T cell with Foxp3 and Tregs as markers, and studying their relationship with TNM classification, differentiated degree, lymphatic metastasis and tumor location. Results The positive rate of Foxp3 and CD8 ^＋T cell in AEG group was significantly higher than that in control group （ P〈0. 05 ） . The distribution of Foxp3 ^＋ in experimental group with lymph node metastasis was higher than that without lymph node metastasis （P〈0. 05）,the positive rate of poorly differentiated was significantly higher than high-differentiated group on Foxp3 （ P〈0. 01 ） , and TNM stage late （Ⅲ） group was significantly higher than earlier TNM stage （Ⅰ-Ⅱ） group （ P=0. 001 ） . The expression of Foxp3 was closely relate with the clinical stages,grades of the masses,the rate of lymphatic metastasis, and significantly higher in group with lymphatic metastasis,poorer differentiation, later stage,while not significantly correlated with the position,age and gender of the patients （ P〉0. 05 ） . Foxp3 was negatively correlated with CD8 in malignant tissues （ r= -0. 314 , P=0. 01） . Conclusion The expression of Foxp3 ^＋ Treg is significantly associated with AEG carcinogenesis and pro-gression. Tregs in AEG tissues possibly promote tumor progression via inhibiting function of CD8 ^＋ T cells.

关 键 词：食管胃交界部腺癌 肿瘤免疫 FOXP3 调节性T细胞 

分 类 号：R730.3[医药卫生—肿瘤]