脑胶质瘤组织中EDNRB基因甲基化状态及其蛋白表达的意义  

Methylation of EDNRB gene and protein expression in glioma

作　　者：成金民[1] 朱立新[2] 王斌[1] 邓鹏程[1] 徐培坤[1] 

机构地区：[1]安徽医科大学第一附属医院神经外科,合肥230022 [2]安徽医科大学第一附属医院中心实验室,合肥230022

出　　处：《安徽医科大学学报》2014年第7期984-987,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省卫生厅基金(编号:2010B013)

摘　　要：目的探讨人脑胶质组织中EDNRB基因的甲基化状态及其蛋白表达的关系。方法采用巢式PCR、亚硫酸氢盐测序PCR（ BSP）及免疫组织化学技术方法，检测50例脑胶质瘤组织标本中EDNRB基因启动子区甲基化状态，分析其甲基化水平、蛋白表达和临床资料之间的相关性。结果脑胶质瘤组织中 EDNRB 基因启动子区甲基化率为38%（19/50），其中低级别组（Ⅰ级-Ⅱ级）中EDNRB基因甲基化率为26.5%（9/34），高级别组（Ⅲ级-Ⅳ级）EDNRB基因甲基化率为62.5%（10/16），两组比较差异有统计学意义（P＆lt;0．05）。而其中甲基化蛋白表达阳性率为36．8%（7/19），未甲基化的蛋白表达阳性率为71%（22/31）。结论组织中的EDNRB基因启动子区CpG岛高甲基化与胶质瘤的发病有关，与胶质瘤的病理级别关系密切，而与患者年龄、性别、肿瘤分型无关；基因甲基化率抑制其蛋白表达。Abstarct Objective To explore the relation between methylation of EDNRB gene and its protein expression in human brain glial tissues. Methods By using nest PCR, bisulfite PCR and immunohistochemistry staining, we investigated the methylation status at EDNRB gene promoter, EDNRB protein expression and their association with patient clinical history. Results In this study, we found EDNRB gene promoter methylation among 38% of total glioma samples（19/50）, with 26. 5% among low grade glioma while 62. 5% among high grade gliomas, which was statistically significant. Within the samples with EDNRB gene methylation events detected, 36. 8% of these samples showed protein expression detected by IHC while 71% samples without EDNRB gene methylation were IHC positive. Conclusion Our study supports the EDNRB gene promoter methylation contributes to gliomagenesis. Moreover, the methylation status correlates with grade of glioma but not with patient age, gender, tumor subtype. The methylation status is negative related to protein expression in gliomas.

关 键 词：脑胶质瘤 内皮素受体B 甲基化 蛋白表达 

分 类 号：R739.1[医药卫生—肿瘤] R730.2[医药卫生—临床医学]