鼻咽癌microRNAs差异表达谱的鉴定  

Idenfication of microRNAs profiles in nasopharyngeal carcinoma

作　　者：聂国辉[1] 刘锋[2] 林宜林 陈静[1] 余振东[1] 李晓庆[3] 段鸿芳 路瑞静 赵春娟[3] 胡洪义[1] 梅雪霜[1] 

机构地区：[1]北京大学深圳医院耳鼻咽喉科,深圳518036 [2]清华大学深圳研究生院 [3]汕头大学医学院 [4]广州医科大学

出　　处：《临床耳鼻咽喉头颈外科杂志》2014年第13期975-978,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：深圳市科技计划项目(No:201208273000135)资助

摘　　要：目的:筛选及验证鼻咽癌中microRNAs(miRs)的差异表达谱。方法:通过芯片高通量表达谱分析及大规模微阵列技术,筛选鼻咽癌与炎症组织中差异表达的miRs,并应用RT-QPCR方法验证筛选结果的可信性。结果:鼻咽癌组织与对照组织的miRs存在差异表达,其中差异倍数大于2倍的人属miRs共有144种。RT-QPCR发现与炎症组织相比,癌组织中miRs-34b、miRs-449b、miRs-7-1表达显著下调,而miRs-125b、miRs-184、miRs-196b、miRs-205及miRs-24-1表达上调,结果与芯片分析结果相一致。结论:差异表达的miRs可能与鼻咽癌的发生、发展密切相关,对鼻咽癌miRs表达谱的研究可能为鼻咽癌的早期诊断及治疗提供有力的靶向依据。Objective：To filtrate and prove the different microRNAs（miRs）profiles in nasopharyngeal carcinoma.Method：Screening the different expressions of miRs between nasopharyngeal carcinoma and the inflammatory tissues by the application of expression profiling of chip high-throughput and large-scale microarray analysis.Then we used RTQPCR technology to prove the accuracy of screening results.Result：There were significant expression differences of miRs between nasopharyngeal carcinoma and the control tissues,144human miRs had 2or more fold the difference ratio.Compared with the inflammatory tissues,we have found that miRs-34b,miRs-449band miRs-7-1significantly low expressed in nasopharyngeal carcinoma,yet miRs-125b,miRs-184,miRs-196b,miRs-205and miRs-24-1expressed high.The results were consistent with the microarray analysis.Conclusion：The difference expressed miRs might be closely related to the process of nasopharyngeal carcinoma,and the research on miRs profiles maybe provide a powerful target basis for early diagnosis and therapy of nasopharyngeal carcinoma.

关 键 词：鼻咽癌 MICRORNAS 表达谱 

分 类 号：R739.63[医药卫生—肿瘤]