形态计量和DNA定量测定在鼻咽癌诊断中的价值  

The diagnostic significance of cellular quantitative morphometry and DNA content measurement in nasopharyngeal carcinoma

作　　者：李琳[1] 李咸龙 张会华 崔英[1] 杨正中 李丽波 

机构地区：[1]昆明市云南省人民医院病理科,昆明医学院病理解剖教研室

出　　处：《耳鼻咽喉（头颈外科）》1995年第4期236-238,共3页Chinese Arch Otolaryngology-Head Neck Surg

摘　　要：应用图像分析技术对７２例鼻咽部取材标本进行了１３项参数及ＤＮＡ定量测定。结果，在Ｉ～Ⅳ组间（Ｉ组正常鼻咽粘膜上皮，Ⅱ组单纯增生或化生，Ⅲ组异型增生或化生，Ⅳ组鼻咽癌），细胞形态参数及ＤＮＡ含量、倍体类型的百分比均值随细胞增生程度而逐渐递变。１３项参数均值进行多元逐步判别分析，优选出４项参数，建立判别函数，回代判别准确率为１００％。ＤＮＡ含量测定以倍体（ｐｌｏｉｄ）表示，认为细胞形态和ＤＮＡ定量分析是一种较为可靠的诊断方法，并提示鼻咽异型增生或化生属癌前病变。Abstract Seventy-two specimens of normal epithlia,hyperplasia/metaplasia,dysplasia/metaplasia and carcinoma of nasopharyngeal mucosa were studied with image analysis technique. 13 pa-rameters of cellular changes and DNA content were measured.The results revealed that,in Ⅰ～Ⅳ groups,morphometric parameter,DNA content and mean percentage of ploid changed with the grade of cellular hyperplasia. The process showed a tendency from quanti-tative to qualitative change. 4 parameters were essentially selected in 13 parameters by step-wise discriminational classification and relating discriminational function was established.An accuracv rate of 100%was achieved. Cellular DNA content presented as ploid.No >5C cell was observed in normal and simple hyperplasia groups. The percentage of >5C cell in na-sopharyngeal carcinoma group reached 10%。This indicated that analysis of cellular mor-phometry and DNA content measurement was a reliable diagnostic method,and dysplasia/metaplasia of nasopharngeal mucosa was part of the precancerous lesions.

关 键 词：鼻咽肿瘤 脱氧核糖核酸 诊断 形态计量 

分 类 号：R739.630.4[医药卫生—肿瘤]