发光菌生物毒性测试方法的改进  

Modification of the Photobacterium phosphoreum Toxicity Test Method

作　　者：林志芬[1] 于红霞[1] 许士奋[1] 王连生[1] 

机构地区：[1]南京大学环境学院国家污染控制与资源化重点实验室,南京210093

出　　处：《环境科学》2001年第2期114-117,共4页Environmental Science

基　　金：国家自然科学基金!(2 9837180 ;2 99770 0 8) ;博士点基金;重点大学基金;日本科学振兴会资助项目

摘　　要：针对发光菌发光强度本底差异较大 ,检测期间发光变化幅度宽的问题 ,在传统发光菌生物毒性测试实验中 ,引入校正因子 (PI丙酮) ,研究了磁力搅拌时间、培养时间、培养代数和培养温度等条件对EC50 测定值的影响 .结果表明改进后的方法实验重现性好 ,相对标准偏差 (RSD)在 2 1 %～ 1 3.1 %之间 .发光菌生物毒性测试方法的改进 ,为获得可靠的毒性实验数据 ,进行多组分有机化合物定量结构 活性相关 (QSARs)的研究奠定了一定的实验基础 .In order to reduce the variation of Photobacterium phosphoreum luminous intensity during testing, the Photobacterium phosphoreum toxicity test was modified by a correction factor, P I acetone ,in this paper. The effects of magnetic stirring time, culture time, culture generation and culture temperature on the measured results were discussed. The modified method had good laboratory repeatability and the relative standard deviation (RSD) was between 2.1% and 13 1%. Reliable data could be obtained using the modified method. It would be beneficial to the development of the quantitative structure\|activity relationships on multicomponent organic compounds.

关 键 词：发光菌 生物毒性 测试方法 校正因子 实验重复性 

分 类 号：X172[环境科学与工程—环境科学] X835.02