柞蚕抗菌肽D转基因酵母的培养及表达产物的分析  

IMPROVED CULTURE METHOD FOR TRANSFER RING ANTIBACTERIAL PEPTIDE GENE FROM CHINESE OAK SILKWORM INTO YEAST AND IDENTIFICATION OF ITS EXPRESSED PRODUCTS

作　　者：马礼金[1] 庄楚雄[1] 黄自然[1] 

机构地区：[1]华南农业大学

出　　处：《蚕业科学》1995年第1期42-46,共5页ACTA SERICOLOGICA SINICA

摘　　要：采用碱金属法（醋酸锂盐），以大肠杆菌－酵母穿梭质粒表达载体在酵母中表达人工合成的柞蚕抗菌肽Ｄ基因。用电泳及抗菌肽活性检测法对表达产物进行分析。同时还研究了转基因酵母的发酵条件，如温度、ｐＨ及营养等对酵母表达的影响。试验结果表明转基因酵母表达产物为１．１４ｍｇ／Ｌ培养液，杀菌活力为０．２１６单位。A synthetic antibacterial peptide gene(APG) from Chinese oak sikworm,Anther-aea pernyi was recombined with a shuttle plasmid pCL WA2 containing an alpha-factorpromotor and transferred into yeast AB103.The cells of yeast grew well under 18-30℃,pH 5.0 in YEPD medium.The expressed products were identified by electrophore-sis with detection of antibacterial activity.The expressed peptide has a molecular weightof 4000 daltons.The yield of product reached a level of 1.14mg/L YEPD and its activityunit is about 0.21 U.

关 键 词：柞蚕 抗菌肽D基因 酵母 

分 类 号：S885.1[农业科学—特种经济动物饲养] S881.2[农业科学—畜牧兽医]