马铃薯优化再生系统的建立  

THE CONSTRUCTION OF OPTIMIZED REGENERATION SYSTEM IN POTATO(SOLANUM TUBEROSUM L.)

作　　者：双宝[1] 李文芙[1] 李文滨[1] 陈伊里[1] 田兴亚[1] 吕文河[1] 王凤义[1] 朱祥春[1] 秦昕[1] 

机构地区：[1]东北农业大学

出　　处：《马铃薯杂志》1995年第3期134-138,共5页Chinese Potato Journal

摘　　要：本试验研究了六种培养基系统对马铃薯东农３０３，Ｆａｖｏｒｉｔａ和极早三个品种来源的不同外植体的愈伤组织诱导和植株再生的影响，筛选出了适合植株再生的最佳外植体、最佳外植体大小及最佳培养基系统。适合植株再生的最佳外植体为叶片；最佳外植体大小为叶片０．５ｃｍ２、茎０．５ｃｍ、块茎０．２５ｃｍ２×０．３ｃｍ，适合于茎、块茎及叶的有效培养基系统分别为：①ＭＳ＋３ｍｇ·Ｌ-１ＢＡ＋０．０１ｍｇ·Ｌ-１ＮＡＡ（ｐｈ５．６）４周ＭＳ＋０．３ｍｇ·Ｌ-１ＧＡ３（ｏＨ５．６）；②ＭＳ＋１．７５ｍｇ·Ｌ-１ＺＴ＋０．８７ｍｇ·Ｌ-１ＩＡＡ（ｐＨ５．８）；③ＭＳ＋２．２５ｍｇ·Ｌ-１ＢＡ＋０．１８６ｍｇ·Ｌ-１ＮＡＡ（ｐＨ５．８）２周ＭＳ＋５ｍｇ·Ｌ-１ＧＡ３＋２．２５ｍｇ·Ｌ-１ＢＡ（ｐｈ５．８）。Six media systems were collected to evaluate the percent of callus formation and shoot regeneration from three potato varieties with their different organs as explants.It was concluded that leaf organ promoted plant regeneration more efficiently than other organs.The size of explants possessed a distinct compact on callus formation and shoot regeneration.The moderate size or three kinds or explants were 0.5cm2 for leaf,0.5cm long for stem and 0.25cm2×0.3cm for tuber.The optimized media systems of plant regeneration for stem,tuber and leaf organs were summarized respectively as:①MS+3mg·L-1BA+0.01mg·L-1NAA(pH=5.6)4 Weeks MS+0.3mg·L-1GA3(pH=5.6);②MS+1.75mg·L-1ZT+0.87mg·L-11AA(pH5.8);③MS+2.25mg·L-1BA+0.186mg·L-1NAA(pH5.8)2WeeksMS+smg·L-1GA3+2.25mg·L-1BA(pH =5.8)

关 键 词：马铃薯 再生系统 再生率 愈伤率 

分 类 号：S532.035.3[农业科学—作物学]