抗肾综合征出血热病毒独特型人源性单克隆抗体的制备  

PRODUCTION OF ANTI-IDIOTYPE HUMAN MONOCLONAL ANTIBODY AGAINST HEMORRHAGIC FEVER VIRUS

作　　者：高磊[1] 陈苏民[1] 陈南春[1] 崔运昌[1] 米力[1] 梁吉 

机构地区：[1]西安第四军医大学

出　　处：《中华传染病杂志》1995年第3期149-151,共3页Chinese Journal of Infectious Diseases

基　　金：国家自然科学基金

摘　　要：分离肾综合征出血热（ＨＦＲＳ）恢复期患者外周静脉血淋巴细胞，经分离的Ｂ淋巴细胞ＥＢ病毒转化后分别与人─鼠种间骨髓瘤细胞系ＳＨＭ－Ｄ３３和小鼠骨髓瘤细胞系Ｘ６３－Ａｇ８．６５３融合，融合率分别为９６％和９０％。杂交瘤生长孔分泌抗ＨＦＲＳＶ抗体阳性率达３３％和２３％。而且均有一孔为抗ＨＦＲＳＶ独特型抗体阳性，经克隆化筛选各得到一株杂交瘤细胞系，命名为Ｃ８和Ｆ３。经鉴定两株杂交瘤细胞系分泌的人源性单克隆抗体均为ＩｇＭ型。Ｆ３抗体产量小于１ｍｇ／ｍｌ，Ｃ８抗体产量为３０～５０ｍｇ／ｍｌ，且已稳定传代两年以上。抗ＨＦＲＳＶ独特型人源性单克隆抗体的成功制备，为ＨＦＲＳＶ疫苗的研制开辟了一条新路。An anti-idiotype human monoclonal antibody against hemorrhagic fever with renalsyndrome virus(HFRSV) was produced by EBV-transformed and fusion method. TheEBV-transformation peripheral blood lymphocytes from the patients with HFRS was fusedto the interspscific myeloma line SHM-D33 or the mouse myeloma linc X63-Ag 8.653.The fusion frequeney of the hybridomas was more than 90%.There were 20～30% ofthe hybridomas secreting human monoclonal antibodies against HFRSV and two clonessecreting anti-idiotype human monoclonal antibodies against HFRSV. One of the celllines(C8 ) produced antibodies at the level of 30～50mg/ml supernatant and has beencontinuously cultured for two years.

关 键 词：人单克隆抗体 出血热病毒 杂交瘤 肾综合征出血热 

分 类 号：R392－33[医药卫生—免疫学] R692[医药卫生—基础医学]