肾癌相关新基因GYLZ-RCC18的表达及意义  

The significance of GYLZ-RCC18,a relatively new gene,in renal cell carcinom

作　　者：张强[1] 张志文[2] 梁丽莉[1] 郭晓健[1] 那彦群[1] 郭应禄[1] 

机构地区：[1]北京大学第一医院,北京大学泌尿外科研究所100034 [2]北京大学医学部生理系

出　　处：《中华泌尿外科杂志》2001年第4期197-199,共3页Chinese Journal of Urology

基　　金：国家自然科学基金资助! (3 9870 841)

摘　　要：目的　探讨GYLZ RCC18功能及在肾癌发生发展中的作用。　方法　应用SMARTRACE技术克隆GYLZ RCC18全长 ,应用逆转录聚合酶链式反应 (RT PCR)特异扩增第 1阅读框中5 0 4bp序列 ,分析在肾癌中的表达特点及与肾癌分级、分期的关系。　结果　GYLZ RCC18基因全长约为 3.5kb ,在肾癌组织特异表达 ,正常肾组织低表达或不表达 ,两者表达量差异约 1～ 9倍。GYLZ RCC18表达在高分级分期肾癌中的表达明显高于低分级分期者。　结论　GYLZ RCC18是肾癌组织相对特异表达的新基因 ,高表达与肾癌的早期发生及晚期进展密切相关。Objective To investigate the role of GYLZ-RCC18 in the generation and development of renal cell carcinoma (RCC). Methods By using SMART RACE and RT-PCR the full length of GYLZ-RCC18 was cloned and its expression was detected in RCC of different grade and stage by amplification of a special fragment sequence in the first reading frame about 504 bp. Results The full length of GYLZ-RCC18 about 3.5 kb expressed in RCC in higher level than in normal kidney tissue.GYLZ-RCC18 overexpressed in high grade and high stage RCC being 1-9 folds higher than that in low grade and low stage ones. Conclusions GYLZ-RCC18 is a new gene especially related to RCC and is closely related to the generation and development of RCC.Study of the gene will be helpful in the understanding of the molecular mechanism of the generation and development of RCC and may direct the diagnosis and treatment of RCC.

关 键 词：肾肿瘤 肾癌 GYLZ-RCC18 基因表达 

分 类 号：R737.11[医药卫生—肿瘤] R730.231[医药卫生—临床医学]