紊乱对猴髁突软骨中TGF-β1分布的影响  

An immunohistochemical study of TGF-β1 in monkey mandibular condyle following gradually induced occlusal disorder

作　　者：陈金武[1] 王美青[1] 姚秀芳[1] 刘豆豆[1] 

机构地区：[1]第四军医大学口腔医学院,西安710032

出　　处：《实用口腔医学杂志》2001年第2期159-168,共10页Journal of Practical Stomatology

基　　金：高等学校骨干教师资助计划资助

摘　　要：目的 :探讨渐进性咬合紊乱所致猴颞下颌关节退形性变中 ,髁突软骨TGF β1的分布变化特点及其意义。方法 :2只青年恒河猴分成实验组和对照组。采用拔牙和固定矫治技术造成实验组后牙渐进性咬合紊乱。 8月后 ,对髁突进行HE染色和TGF β1免疫组化定量分析。 结果 :①实验组髁突前内侧出现明显的退性形变 ,与对照猴相比 ,其髁突各部位软骨厚度均变薄 (P <0 .0 1) ,其中变化程度最大者为前部内份 ,其次是髁突前部中份和中部内份。②与对照猴相比 ,实验组髁突软骨各部位TGF β1表达均升高 (P <0 .0 1)。结论 :渐进性咬合紊乱可导致髁突退性形变 ,髁突软骨中TGF β1表达明显增强 ,TGF β1可能参与了关节软骨的修复活动。砄bjective:To elucidate the effects of gradually induced occlusal disorder on the expression of TGF β1 in temporomandibular condyle of monkey. Methods:In a monkey after extraction of right upper first molar and left lower first molar, a fixed orthodontic appliance was used to cause the adjacent teeth inclined to the space and to occlude with opposite in coordinately. Another monkey was used as the control.8 months later, the animals were sacrificed and bilateral temporomandibular condyles were examined with histologic and immunohistochemical methods.Results:In the animal with teeth extraction, degenerative changes were found in anterior medial part of condyles, the thickness of condylar cartilage decreased significantly, especially in the anterior parts of medial and central thirds, and also the intermediate part of medial third ( P < 0.01 ). A significant increase of TGF β1 in condylar cartilage was found when compared with normal control ( P <0.01).Conclusion:Gradually induced occlusal disorder can lead to degenerative changes in monkey temporomandibular joint with increased TGF β1 expression in condylar cartilage.TGF β1 may participate in cartilage repairing during the procedure.

关 键 词：颞下颌关节紊乱 转化生长因子Β 髁突软骨 He紊乱 

分 类 号：R782.6[医药卫生—口腔医学]