以羟基喜树碱为基础的联合方案对实验性鼻咽癌抗瘤作用的研究  

Study of Anticancer Effect of Hydroxycampothecin- based Combination against Experimental Nasopharyngeal Carcinoma

作　　者：符立梧[1] 梁永钜[1] 潘启超[1] 

机构地区：[1]中山医科大学肿瘤防治中心,广东广州510060

出　　处：《癌症》2001年第4期363-367,共5页Chinese Journal of Cancer

基　　金：国家自然科学基金项目!( NO.39770840)

摘　　要：目的：探讨以拓扑异构酶 I抑制剂羟基喜树碱（ 10- hydroxycampothecin,HCPT）为基础的联合方案对实验性鼻咽癌 (nasopharyngeal carcinoma,NPC)的抗瘤作用。方法：以人 NPC细胞株 CNE2的体外和裸鼠移植瘤为模型，研究 HCPT与顺铂（ cisplatin,DDP）或（和） 5-氟尿嘧啶（ 5- fluorouracil,5- FU）联合作用的抗瘤效果。细胞毒测定用 MTT法，体内抗瘤模型为 CNE2裸鼠移植瘤模型。结果： HCPT、 DDP和 5- FU对 CNE2的 IC50分别为 14.2、 17.2及 49.2μ mol/L。 HCPT与 DDP合用具有明显的协同抗 NPC作用。 HCPT与 5- FU合用虽较单药抗瘤作用优，但未见明显的相加或协同作用。 HCPT＋ DDP＋ 5- FU三药合用较两药合用效果更优，且毒性可以耐受。结论： HCPT＋ DDP或 HCPT＋ DDP＋ 5- FU具有协同抗 NPC肿瘤作用，可试用于临床。Objective: This study was designed to evaluate topoisomerase I inhibitor 10- hydroxycampothecin- based combination chemotherapy for nasopharyngeal carcinoma (NPC) in vitro and in vivo. Methods: Antitumor activity of 10- hydroxycampothecin (HCPT) in combination with 5- fluorouracil (5- FU) and/or cisplatin (DDP) in NPC cell line CNE2 and in CNE2 xenograft of nude mice were determined. The cytotoxicity was measured by MTT assay. Results: HCPT, 5- FU,and DDP had potential cytotoxicity to CNE2 cells. Their IC50 were 14.2μ mol/L, 17.2μ mol/L, 49.2μ mol/L,respectively. HCPT in combination with DDP had significant anticancer effect for NPC in vitro and in vivo. But no significant additivity or synergism was showed in the combination of HCPT with 5- FU in vitro and in vivo. HCPT＋ DDP＋ 5FU was much better than HCPT＋ DDP for chemotherapy of NPC and the toxicity was endurable in the nude mice bearing CNE2 xenograft. Conclusion: HCPT＋ DDP and HCPT＋ DDP＋ 5- FU were potential regimen for chemotherapy of NPC.

关 键 词：拓扑异构酶Ⅰ抑制剂 羟基喜树碱 顺铂 5-氟尿嘧啶 鼻咽肿瘤 实验 

分 类 号：R739.63[医药卫生—肿瘤] R73－361[医药卫生—临床医学]