增强子样序列对不同类型启动子作用的研究  

Study on the Effect of A Bacterial Enhancer-Like Element (BELE) on the Different Types of Promoters

作　　者：关国晟 陈次滨 陈安薇 张求旺[2] 吴淑华[2] 

机构地区：[1]广州市第二人民医院,广州510150 [2]广州医学院,广州510182

出　　处：《现代临床医学生物工程学杂志》2001年第1期14-15,共2页Journal of Modern Clinical Medical Bioengineering

摘　　要：目的　探讨大肠杆菌谷氨酰胺合成酶基因上游增强子样序列 (BELE)有无严格的启动子选择性 .方法　人工合成 5 1bp的BELE插入质粒PATNF15 3并位于trp启动子和肿瘤坏死因子基因上游 ;在另一质粒PAL -B中 ,BELE插入痘苗病毒 7.5K蛋白基因启动子和β -半乳糖苷酶基因上游 ,测定TNF及Lac基因的生物学活性 . 结果　活性测定结果表明BELE对上述两种启动子的转录作用有增强效应 .Objective To investigate if a bacterial enhancer-like element (BELE) in the upstream region of Escherichia coli gln Ap2 gene had strict promoter selectivity.Methods 51 bp fragment of BELE was synthesized and inserted into the plasmid PATNF153,locating upstream trp promoter (sigma 70) and TNF gene,in the other plasmid PAL-B,BELE was inserted into the upstream of Vaccinia Virus 7.5K promoter (sigma?) and lac gene. We tested the biological activity of TNF gene and lac gene based on the transient expression.Results The results indicate that both promoters could be enhanced a high level of transcriptional activity by BELE.Conclusions BELE has not strict promoter selectivity.

关 键 词：原核增强子 启动子 肿瘤坏死因子 Β-半乳糖苷酶基因 

分 类 号：R394.3[医药卫生—医学遗传学]