维甲酸抑制人胃癌细胞株层粘素受体基因和原癌基因C-Ha-ras mRNA的表达  

The Inhibitory Effect of Retinoic Acid on the Expression of Laminin Receptor Gene and Protooncogene C-Ha-ras mRNA in Human Gastric Cancer Cell Line

作　　者：孙月霞[1] 许伟榕[1] 朱敏[1] 何震平[1] 陈诗书[1] 

机构地区：[1]上海第二医科大学生化教研室,,200025

出　　处：《胃肠病学》2001年第1期14-16,共3页Chinese Journal of Gastroenterology

摘　　要：目的：研究维甲酸（ＲＡ）对人胃癌细胞株（ＳＧＣ７９０１）层粘素受体（ＬＮＲ）基因和原癌基因Ｃ－Ｈａ－ｒａｓ表达的影响。方法：应用原位杂交方法标记ＬＮＲ探针和Ｃ－Ｈａ－ｒａｓ探针，对人胃癌细胞（分为经ＲＡ处理及未经ＲＡ处理两组）中ＬＮＲ和Ｃ－Ｈａ－ｒａｓ基因的ｍＲＮＡ表达进行检测。结果：经ＲＡ处理的人胃癌细胞ＬＮＲ ｍＲＮＡ表达较未经ＲＡ处理者显著降低（Ｐ＜０．０１～０．００１）；Ｃ－Ｈａ－ｒａｓ转录水平亦显著降低（Ｐ＜０．０１～０．００１）。结论：ＲＡ可显著抑制人胃癌细胞ＬＮＲ和Ｃ－Ｈａ－ｒａｓ基因的表达。Background/Aims: To investigate the expression of laminin receptor (LNR) gene and protooncogene C-Ha-ras in human gastric cancer cell line (SGC7901) induced differentiation with retinoic acid (RA). Methods: The cell hybridization was used to detect the mRNA expression of LNR and C- Haras gene of SGC7901 treated/untreated with RA. Results: The mRNA expression levels of LNR and CHa-ras were significantly decreased in the cells treated with RA than those untreated ones (P<0.01- 0.001). Conclusions: RA could highly inhibit the expression of both LNR and C-Ha-ras gene in human gastric cancer cells.

关 键 词：胃肿瘤 细胞系 维甲酸 层粘连蛋白受体 RAS基因 原癌基因 P2蛋白 mRNA 

分 类 号：R735.2[医药卫生—肿瘤] R730.231[医药卫生—临床医学]