AC133抗原在急性白血病细胞中的表达  

Expression of AC133 Antigen on Acute Leukemia Cells

作　　者：于弘[1] 常艳[1] 刘艳荣[1] 付家瑜[1] 陈珊珊[1] 

机构地区：[1]北京大学人民医院血液病研究所,北京100044

出　　处：《中国实验血液学杂志》2001年第1期26-29,共4页Journal of Experimental Hematology

摘　　要：为了解AC133抗原在急性白血病细胞中的表达情况及其临床意义 ,采用常规三色直接免疫荧光标记法 ,使用流式细胞仪测定AC133抗原及其它白血病相关抗原在 61例急性白血病患者细胞中的表达。检测结果显示 ,61例中有 2 7例 (44 .3% )AC133表达阳性 ,其中ALL 13例 (13 2 9) ,AML M0 M16例 (6 8) ,AML M2 4例 (4 16) ,AML M4 M54例 (4 8)。 2 7例AC133+病例中2 5例 (92 .6% )同时表达CD34,13例 (48.1% )同时表达CD117。本研究结果提示 ,AC133抗原与CD34和CD117的表达有相关性。AC133抗原可表达于各型白血病中 ,但在AML M0To investigate the expression of AC133 antigen on acute leukemia cells and its clinical significance, samples of 29 patients with acute lymphocytic leukemia (ALL) and 32 patients with acute myelocytic leukemia (AML) were analyzed by flow cytometric three-color direct immunofluorescence methods. It was found that 13 of 29 ALL, 6 of 8 AML-M 0/M 1, 4 of 16 AML-M 2 and 4 of 8 AML-M 4/M 5 expressed AC133. The mean fluoresence intensity of AC133 in each subtype of AML-M 0/M 1, AML-M 2 and AML-M 4/M 5 was 227.1, 143.1 and 142.5, respectively. Among 27 AC133 + cases (44.3%), 25 cases coexpressed CD34 (92.6%) and 13 cases coex- pressed CD117 (48.1%). In contrast to other reports, our data do not support the findings that AML-M 4/M 5 expressed very high levels of AC133. In conclusion, AC133 antigen, coexpressed with CD34 and CD117, appears more frequently and more intensely in AML-M 0/M 1.

关 键 词：急性白血病 AC133抗原 CD34 CD117 表达 

分 类 号：R733.710.3[医药卫生—肿瘤]