rhIL-11对照射所致骨髓抑制猕猴的治疗作用(英文)  

Therapeutic Effects of rhIL-11 on Irradiation-Induced Myelosuppression in Rhesus Monkeys

作　　者：郝静[1] 罗庆良[1] 熊国林[1] 柳晓兰[1] 邱丽玲[1] 陈国志[1] 黄缨[1] 葛平[1] 耿煜[1] 孙连生[1] 董波[1] 李元敏[1] 陈望秋 沈纯 

机构地区：[1]北京放射医学研究所,北京100850 [2]医进生物技术(北京)有限公司,北京100875

出　　处：《中国实验血液学杂志》2001年第1期56-61,共6页Journal of Experimental Hematology

基　　金：全军"九五"指令性课题基金资助 编号 960 5 0 0 1

摘　　要：本研究观察了rhIL 11对γ线照射后猕猴血小板和白细胞减少症的治疗作用 ,同时比较了不同时间给药对疗效的影响。用6 0 Coγ射线 3.0Gy照射正常猕猴 ,造成骨髓抑制模型。照射后当天开始给予rhIL 1130 ,60或 12 0 μg·kg-1·day-1,连续 14天皮下注射 ,可以显著提高给药猴外周血小板数的最低值 ,虽然白细胞数最低值并未上升 ,但低于照前值 5 0 %的时间明显缩短 ,这与血小板的变化相似。照射后当天开始给予rhIL 11治疗的动物在照射后开始的 2周内红细胞数较对照组略有下降 ,但第 3周即迅速上升并超过对照组。照射后第 13天开始给予rhIL 11治疗的 4只猴 ,在照射后前 3周内外周血象与对照组相似 ,此后迅速恢复。骨髓细胞培养结果表明 ,rhIL 11治疗猴的骨髓细胞在体外形成CFU Meg ,CFU Mix ,CFU E ,BFU E和CFU GM能力明显增加。照射后第 4 5天病理组织学检查结果表明 ,照射对照组动物骨髓腔空虚并有明显出血 ,治疗组动物骨髓腔中细胞丰富 ,生长旺盛。这些结果表明 ,rhILThe efficacy of rhIL-11 in treating thrombocytopenia and neutropenia in γ-irradiated rhesus monkeys and the variation in curative effect due to difference of administration times were studied. Healthy rhesus monkeys were exposed to 3.0 Gy 60Co total body irradiation (TBI) to result in pancytopenia for three weeks. Treatment with rhIL-11 (30, 60 or 120 μg·kg -1·day -1) on early days (days 0-13 after TBI) could significantly improve the nadir of platelet count. Although the nadir of leukocyte count was not improved, the duration below 50% of its baseline value was shortened similarly to that of platelet. During the first two weeks after TBI, erythrocyte numbers of the animals treated with these doses of rhIL-11 were lower than those of the control group at first but they became higher beginning from the third week. Four monkeys were treated with rhIL-11 at 60 μg·kg -1·day -1 on days 13-26 after TBI. The numbers of their peripheral blood cells followed the similar decrease patterns as those of control group during the first three weeks, then they were improved rapidly. By semi-solid bone marrow cell culture it was demonstrated that rhIL-11 could stimulate bone marrow cells to form more CFU-Meg, CFU-Mix, CFU-E, BFU-E and CFU-GM in vitro. Histopathological observation revealed that bone marrow of the control group was devoid of hematopoietic cells and bleeding, being contrary to that of the group treated with rhIL-11, in which the cells proliferated actively. The results suggest that rhIL-11 can accelerate hematopoietic recovery of irradiated monkeys.

关 键 词：造血细胞因子 重组人白细胞介素-11 照射 骨髓抑制 猕猴 

分 类 号：R730.55[医药卫生—肿瘤] R558.205[医药卫生—临床医学]