人肺癌细胞系HB-99的建立及其生物学特征  

Establishment and Characterization of Human Lung Carcinoma Cell Line HB-99

作　　者：黄昀[1] 吴焱[1] 杨焕杰[1] 张临友[2] 傅松滨[1] 刘权章[1] 

机构地区：[1]哈尔滨医科大学医学遗传学教研室,哈尔滨150086 [2]哈尔滨医科大学附属第二医院胸外科,哈尔滨150086

出　　处：《遗传》2001年第2期103-106,共4页Hereditas（Beijing)

基　　金：黑龙江省科学技术计划攻关;青年科学技术攻关项目!(G99C20-6-1)资助

摘　　要：利用一例肺鳞癌手术标本通过原代培养建立了肺癌细胞系命名为HB-99。该细胞系呈单层贴壁生长，从相差显微镜和电镜分析具有细胞的多形性，细胞倍增时间为24小时，克隆形成率40%，染色体改变复杂，众数63-65。将细胞移植到裸鼠体内而生长的肿块具有与原始病人手术标本相似的组织形态。免疫组织化学分析，近100%的细胞表达角蛋白17（CK17），10%的细胞表达波形蛋白（vimentin）。根据该细胞系的生物学特征提示HB-99是一新建立的肺鳞癌细胞系。We have established a human lung squamous carcinoma cell line ,designed HB-99, by culturing primary tumor sample. The cells of HB-99 derived from resected specimen of a male patient with lung squamous cancer. The y grew in monolayers and showed cellular morphology by phase contrast and electr onic microscopy. The HB-99 cells had a doubling time of 24 hours and a cloning efficiency of 40%. Chromosomal analysis showed complicated rearrangements with a modal number of 63～65. When hetero-transplanted to nude mice, HB-99 grew to fo rm tumor with the same morphology as the original one from the patient. The resu lts of immunohistochemistry suggested that CK17 expressed in almost all cells wh ile only 5%～10% cells had Vimentin. HB-99 is really a newly established cell li ne of lung squamous carcinoma.

关 键 词：肺癌 细胞系 生物学特征 人 肺肿瘤 

分 类 号：R734.2[医药卫生—肿瘤]