枯草芽孢杆菌中性植酸酶的纯化和酶学性质  

PURIFICATION AND PROPERTIES OF NEUTRAL PHYTASE FORM \%BACILLUS SUBTILIS'

作　　者：王亚茹[1] 姚斌[1] 曾虹[1] 史秀云[1] 操时树[1] 袁铁铮[1] 范云六[2] 

机构地区：[1]中国农业科学院饲料研究所 [2]中国农业科学院生物技术研究所,北京100081

出　　处：《微生物学报》2001年第2期198-203,共6页Acta Microbiologica Sinica

基　　金：国家"863"高技术计划资助项目!( 1 0 1 0 3 0 5 0 1 )&&

摘　　要：从土壤中分离到了产中性植酸酶的枯草芽孢杆菌菌株并对所产植酸酶进行了分离纯化。此中性植酸酶的反应最适 pH为 7 5,最适温度为 55℃ ,在 37℃下以植酸钠为底物的Km值为 0 1 9mmol/L ,植酸酶活性依赖Ca2 +的存在。酶蛋白的分子量大小约为 45kD ,纯酶蛋白N端序列为Lys His Lys Leu Ser Asp Pro Tyr His Phe Thr。A strain Bacillus subtilis producing neutral phytase was screened from soil.The protein of phytase was purified by HPLC.Optimal pH value and temperature of the phytase for its activity were 7.5 and 55℃,respectively.The K m values of the phytase for dodecasodium phytate under 37℃ was 0.19mmol/L.The molecule weight of the phytase protein was determined as about 45kD by SDS\|PAGE.The N\|terminal amino acids sequence of the phytase protein was determined as Lys\|His\|Lys\|Leu\|Ser\|Asp\|Pro\|Tyr\|His\|Phe\|Thr by amino acids sequence analysis.

关 键 词：枯草芽孢杆菌 中性植酸酶 酶学性质 纯化 

分 类 号：Q936[生物学—微生物学] Q55