力达霉素对人结肠癌HCT-8细胞侵袭调节基因表达的影响  

EFFECT OF LIDAMYCIN ON THE EXPRESSION OF GENES INVOLVED IN INVASION REGULATION IN HCT-8 HUMAN COLON CANCER CELLS

作　　者：崔大鹏[1] 王真[1] 李电东[1] 

机构地区：[1]中国医学科学院,中国协和医科大学医药生物技术研究所,北京100050

出　　处：《药学学报》2001年第4期246-249,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目! (30 0 70 878) ;国家重点基础研究发展规划资助项目 !(G2 0 0 0 0 5 70 10 )

摘　　要：目的　研究抗肿瘤抗生素力达霉素对人结肠癌HCT 8细胞侵袭调节基因表达的影响。方法　利用cDNA排列 (cDNAarrays)、Northern杂交、RT PCR技术。结果　力达霉素可以促进TIMP 1的表达 ,抑制MMP 9的表达 ,cDNA排列、Northern杂交、RT PCR检测得到了同样的结果。结论　力达霉素可能通过抑制IV型胶原酶的产生 ,同时诱导金属蛋白酶抑制因子的产生 。AIM To study the effect of lidamycin on the expression of genes involved in invasion regulation in HCT-8 human colon cancer cells. METHODS HCT-8 human colon cancer cells were treated with lidamycin (10 nmol·L -1 ) for 8 h. The effect of lidamycin on the expression of genes were detected by cDNA arrays, Northern blot and RT-PCR. RESULTS Hybridization of the entire cDNA populations to Atlas Arrays showed that lidamycin down-regulated the expression level of MMP-9 and up-regulated the expression level of TIMP-1. These changes were confirmed by Northern blot and RT-PCR. CONCLUSION The results indicate that lidamycin may exhibit its anti-invasive activity by inhibitting the production of type IV collagenase whilst enhancing the production of tissue inhibitor metalloproteinase.

关 键 词：抗肿瘤抗生素 力达霉素 cDNA排列 基质金属蛋白酶 基质金属蛋白酶抑制因子 结肠癌 

分 类 号：R979.14[医药卫生—药品] R735.35[医药卫生—药学]