免疫佐剂增强乙型肝炎核心抗原核酸疫苗免疫应答的小鼠初步实验  

Adjuvant QS-21 enhances immune responses in mice induced by DNA vaccine based on core antigen of hepatitis B virus

作　　者：黄祖瑚[1] 刘宁[1] 李军[1] 唐保元[1] 卢山[2] 王世霞[2] 

机构地区：[1]南京医科大学第一附属医院,210029 [2]美国马萨诸塞大学医学中心

出　　处：《中华传染病杂志》2001年第1期8-10,共3页Chinese Journal of Infectious Diseases

基　　金：卫生部科学研究基金资助项目!(96 1347)

摘　　要：目的　观察小鼠实验中免疫佐剂QS 2 1对乙型肝炎核心抗原 (HBcAg)核酸疫苗免疫应答的增强作用。方法　基因枪轰击法接种HBcAg核酸疫苗或对照质粒 ,皮内注射法给予QS 2 1;间接酶联免疫吸附试验 (ELISA)法检测小鼠血清抗 HBc(IgG及IgG亚类 )水平 ;51铬释放法检测小鼠脾细胞特异性CTL杀伤活性。结果　Balb/c和C5 7BL/ 6小鼠单用疫苗组和疫苗加QS 2 1组的血清抗 HBc终点滴度均分别为 1∶32 80 5 0和 1∶98415 0 ;两组的抗 HBc IgG亚类均以IgG2a略占优势 ;当效∶靶比为 12∶1时 ,Balb/c小鼠单用疫苗组和疫苗加QS 2 1组的特异性CTL杀伤活性分别为 5 1.1%和6 3 .6 % ;C5 7BL/ 6小鼠两组的CTL杀伤活性分别为 5 3 .1%和 6 8.1%。结论　QS 2Objective To observe the effect of adjuvant QS 21 on immune responses in mice induced by DNA vaccine based on core antigen of hepatitis B virus (HBcAg). Methods Gene gun bombardment technique was used for vaccinating DNA vaccine of HBcAg or control plasmid. Adjuvant QS 21 was given through intradermal injection. Anti HBc (IgG) and its isotypes (IgG1, IgG2a) in mice sera were detected by ELISA. HBcAg specific cytotoxic T lymphocyte (CTL) activity was measured by 51 Chromium release assay. Results In both C57BL/6 (H 2 b) and Balb/c (H 2 d) mice, the end point dilution titers of anti HBc IgG in the group receiving DNA vaccine alone and the group receiving DNA vaccine+QS 21 were 1∶328050 and 1∶984150, respectively. IgG2a, an isotype of anti HBc IgG, was slightly higher than IgG1 in all groups of mice, suggesting that Th1 type of helper T cell response was activated. In C57BL/6 (H 2 b) mice, HBcAg specific CTL lysis activities in the group receiving DNA vaccine alone and the group receiving DNA vaccine +QS 21 were 51.1% and 63.6% at the E∶T ratio of 12∶1, while in Balb/c (H 2 d) mice the corresponding data for HBcAg specific CTL lysis activities were 53.1% and 68.1%, respectively. Conclusions Adjuvant QS 21 is effective in enhancing both humoral immune responses and CTL activity in mice immunized with DNA vaccine of HBcAg.

关 键 词：免疫佐剂 乙型肝炎病毒 DNA疫苗 小鼠 免疫应答 

分 类 号：R575.1[医药卫生—消化系统]