人乳头瘤病毒16型(湖北株)E7蛋白在NIH/3T3细胞中的表达和定位  

Expression and localization of Hubei strain type HPV16 E7 protein in NIH/3T3 cells

作　　者：盛德乔[1] 伍欣星[2] 赵文先[2] 

机构地区：[1]三峡大学医学院生物化学教研室,宜昌443003 [2]湖北医科大学病毒研究所分子生物室,武汉430071

出　　处：《西安医科大学学报》2001年第1期5-6,33,共3页Journal of Xi'an Medical University(Chinese)

基　　金：湖北省科委重大科研项目经费;湖北省自然科学基金资助

摘　　要：目的　将带有人乳头瘤病毒 1 6型 (湖北株 )E7基因的真核表达载体 pL(E7 HB)SN导入NIH/3T3细胞进行瞬时表达。方法　重组质粒转染 3T3细胞通过DNA 磷酸钙共沉淀法 ;E7基因表达的检测采用RT PCR和间接免疫荧光实验。结果　E7基因导入真核细胞后能有效转录并表达E7蛋白 ,通过免疫荧光染色可看到E7蛋白主要定位在胞浆中 ,细胞核中也有 ,但量较少。结论　这可能和湖北株HPV1 6E7基因发生突变有关。Objective The eukaryotic plasmid pL(E7 HB)SN with HPV16 E7 gene(Hubei strain) was transfected into NIH/3T3 cells for transient expression.Methods The recombinant plasmid was transfected into NIH/3T3 by calcium phosphate DNA coprecipitation.The expression of E7 gene was examined by RT PCR and the indirect immunofluorescence.Results It was found that the E7 gene could transcript and express the E7 protein efficiently. And the E7 protein could be seen predominantly in cytoplasm,but it was few in nucleus.Conclusion This phenomenon may be correlated with the mutation of E7 gene.

关 键 词：人乳头瘤病毒 定位 HPV16 E7基因 基因表达 肿瘤 NIH/3T3细胞 重组质粒 

分 类 号：R730.3[医药卫生—肿瘤]