多重PCR技术检测宫颈癌组织中HPV16、18和11/6E_6基因的研究  

E_6 genes of human papilloma virus 16,18,11/6 in cervical carcinoma detected by multiple polymerase chain reaction

作　　者：陈晓[1] 周宜男[1] 张月明[2] 赵富玺[3] 

机构地区：[1]新疆医科大学基础医学院病理学教研室,山西大同037008 [2]新疆医科大学基础医学院营养与癌研究室,新疆乌鲁木齐830054 [3]山西大同医学专科学校微生物学教研室,山西大同037008

出　　处：《新疆医科大学学报》2001年第1期18-20,共3页Journal of Xinjiang Medical University

摘　　要：目的 :研究宫颈癌发病与 HPV16、18E6基因的关系。方法 :采用多重 PCR技术检测 83例宫颈癌组织中 HPV16、18及 11/ 6 E6基因。结果 :( 1)新疆地区 36例宫颈癌中 HPV E6基因检出率为 86 .11% ( 31/ 36 ) ,其中HPV16 E6占构成比 80 .5 6 % ( 2 5 / 31)。 ( 2 )山西地区宫颈癌中 HPV DNA阳性检出率 5 1.0 6 % ( 2 4/ 47) ,其中HPV16 E6占构成比 5 0 .0 0 % ( 12 / 2 4)。 ( 3)两组 HPV E6阳性检出率比较 P <0 .0 1,差异有显著性。两组 HPV16E6构成比比较 ,P >0 .0 5 ,差异无显著性。结论 :宫颈癌发生与 HPV感染的关系密切 ,且新疆地区比山西地区更为密切。两组 HPV感染以Objective: To study the relationship between the occurrence of cervical carcinoma and E\-6 genes of HPV 16, 18, 11/6 distributed in different districts. Methods: E\-6 genes of HPV 16, 18, 11/6 were detected using the multiple polymerase chain reaction on formalin fixed, paraffin embeded material in 83 cases of cervical carcinoma. Results: ①The positive rate of E\-6 genes of HPV 16,18,11/6 in 36 cases in Xinjiang was 86.11%(31/36). The proportion of HPV 16 E\-6 gene was 80.56%(25/31). ②The positive rate of E\-6 genes of HPV 16,18,11/6 in 47 cases in Shanxi was 51.06%(24/47). The proportion of HPV 16 E\-6 genes was 50.00%(12/14). ③The positive rates of two groups through chi square test revealed clear difference (P<0.01),however, the proportions of HPV 16 E\-6 genes between two groups were no significant difference (P>0.05). Conclusions: The occurrence of cervical carcinoma may be closely related to the infection of HPV especially in Xinjiang .The infection of HPV type 16 may be the most important etiologic factor in both groups.

关 键 词：人类乳头状瘤病毒 多重聚合酶链反应 宫颈癌 HPV16 HPV18 11/6E6基因 

分 类 号：R737.33[医药卫生—肿瘤] R730.2[医药卫生—临床医学]