Dieldrin对PC12细胞的增殖及酪氨酸羟化酶mRNA表达的影响  

Effects of Dieldrin on the Proliferation of PC12 Cell and the Expression of Tyrosine Hydroxylase mRNA

作　　者：彭湘闽[1] 任惠民[1] 蒋雨平 

机构地区：[1]复旦大学附属华山医院神经科,200040

出　　处：《中国临床神经科学》2001年第1期9-12,共4页Chinese Journal of Clinical Neurosciences

基　　金：国家教委博士点科学研究基金课题 (9831 )

摘　　要：目的 :观察有机氯杀虫剂dieldrin对培养的PC12细胞增殖及对其酪氨酸羟化酶mRNA(THmRNA)表达的影响 ,探讨该毒物致帕金森病 (PD)的可能机制。方法 :以MPP+ 为阳性对照 ,用不同浓度 (10 -4 ～ 1mmol·L-1)的MPP+ 和dieldrin分别于预PC12细胞 2 4h后计细胞数 ,并采用半定量逆转录聚合酶链式反应 (RT PCR)方法测定THmRNA表达的改变。结果 :(1)MPP+ 和dieldrin对PC12细胞均有毒性 ,1mmol·L-1的MPP+ 可使细胞数减少至对照组的 45 % (P <0 0 1) ,低于此浓度的MPP+ 对细胞增殖无影响 ;但 0 1mmol·L-1的dieldrin就可使细胞数减少至对照组的 3 0 % (P <0 0 1) ,1mmol·L-1的dieldrin使PC12细胞全部死亡。 (2 )MPP+ 和dieldrin均可降低THmRNA的表达。 0 1mmol·L-1MPP+ 和dieldrin使细胞THmRNA的表达分别下降至对照组的 69 3 % (P >0 0 5 )和 61 7% (P <0 0 1)。结论 :Dieldrin不仅具有与MPP+ 相同的抑制培养的PC12细胞的增殖及THmRNA表达的作用 ,而且其对细胞的毒性强于MPP+ ,提示dieldrin是一种潜在的致PD物质 。Aim:To evaluate the possible pathogenesis of dieldrin by measuring the PC12 cell survival and the expression of tyrosine hydroxylase mRNA (THmRNA) after incubation with dieldrin for 24h.Methods:PC12 cells were selected in this study. MPP + was used as a positive control. After 24h of growth, various concentrations of MPP + and dieldrin (10 -4 ～1 mmol·L -1 ) were added to the growth medium for 24h. PC12 cell survival was examined and the expression of THmRNA was measured by using RT PCR. Results:(1) Both MPP + and dieldrin had toxic effects on PC12 cell. The PC12 cell survival significantly decreased to 45 % of the control after incubation with 1 mmol·L -1 MPP + for 24h. However, MPP + had no effect on cell survival when its concentration was lower than 1 mmol·L -1 . The PC12 cell survival significantly decreased to 30 % ( P <0 01) of the control after incubation with 0.1 mmol·L -1 dieldrin for 24 h. Morever, after treating with 1 mmol·L -1 dieldrin for 24h, the PC12 cells were totally dead. (2) Both MPP + and dieldrin had toxic effects on the expression of THmRNA. When PC12 cells were incubated with 0.1 mmol·L -1 MMP + and dieldrin for 24 h, the expressions of THmRNA were decreased to 69.3% ( P >0 05) and 61.7% ( P <0 01) of the control,respectively. Conclusion:Dieldrin, which has stronger toxic effects on PC12 cell survival and the expression of THmRNA than MPP +,may be a potential etiological agent for parkinsonism.

关 键 词：帕金森病 DIELDRIN MPP^+ 酪氨酸羟化酶mRNA PC12细胞 

分 类 号：R742.502[医药卫生—神经病学与精神病学]