系统性红斑狼疮患者外周血单一核细胞NF-κB信号通路活化检测  

Activation of NF－κ B Signaling Pathway in Peripheral Blood Mononuclear Cells of Patients With Systemic Lupus Erythematosus

作　　者：许韩师[1] 叶任高[1] 孙林[1] 杨琼琼[1] 曾丽霞[1] 李志坚[1] 李幼姬[1] 

机构地区：[1]中山医科大学附属第一医院肾内科,广州510080

出　　处：《中华皮肤科杂志》2001年第1期18-21,共4页Chinese Journal of Dermatology

基　　金：中山医科大学 211工程重点项目资助课题 !(98150)

摘　　要：目的检测系统性红斑狼疮 (SLE)患者外周血单一核细胞 (PBMC) NF－κ B信号通路活化情况 ,并探讨其临床意义。方法 NF－κ B活性检测采用电泳迁移率改变试验 , Iκ Bα蛋白及其磷酸化产物采用蛋白印迹 ,抗 dsDNA抗体、 IgG、 IgM测定采用 ELISA方法。结果 SLE患者 PBMC NF－κ B活性显著高于正常对照组 (P< 0.05),活动期明显高于缓解期 (P< 0.05), SLE组 Iκ Bα蛋白表达显著低于正常对照组（ P< 0.05） ,活动期患者低于缓解期 (P< 0.05)。 SLE组 Iκ Bα磷酸化产物显著高于正常对照组（ P< 0.05） ,活动期患者高于缓解期 (P< 0.05)。 NF－κ B活性和 Iκ Bα磷酸化产物均与抗 dsDNA抗体、 IgG和 SLE疾病活动指数呈正相关 ,而与 IgM无相关关系。结论 SLE患者 PBMC存在 NF－κ B信号通路异常活化 ,且与抗 dsDNA抗体、 IgG分泌密切相关。检测 SLE PBMC内 NF－κ B信号通路蛋白表达可能有助于 SLE疾病活动的判断。Objective To detect the activation of NF－κ B signaling pathway in peripheral blood mononuclear cells(PBMCs) of patients with systemic lupus erythematosus(SLE) and investigate its clinical significance. Methods Activation of NF－κ B was detected by electrophoretic mobility shift assay( EMSA), the expression of Iκ Bα protein and its phosphorylated products were detected by Western blot. Anti-dsDNA antibody, IgG and IgM in supernatant of PBMC culture were tested by ELISA. Results (1)Elevated activation of NF－κ B in PBMC of SLE patients was found when compared with the controls(P< 0.05), and activation of NF－κ B in active SLE group was higher than that of inactive SLE group(P< 0.05). (2) The expression of Iκ Bα in SLE group was lower than that of the control group(P< 0.05), and Iκ Bα in active SLE group was lower than that of inactive SLE group(P< 0.05).(3) Iκ Bα phosphorylated product in SLE group was higher than that in the control group, and the product in the active SLE group was increased compared with the inactive SLE group.(4)The activation of NF－κ B and Iκ Bα phosphorylated product had a positive correlation with the titer of anti-dsDNA antibody, IgG in the supernatant of PBMC culture and SLEDAI, respectively. Conclusions There are abnormal activation of NF－κ B signaling pathway in SLE. Detection of activation of NF－κ B signaling pathway in PBMC may be used as a predicator of SLE disease activity.

关 键 词：系统性红斑狼疮 单核白细胞 NF-KB IKBΑ 信号通路 SLE 

分 类 号：R593.241[医药卫生—内科学]