DEN诱发大鼠肝癌变的病理形态与细胞增殖活性的定量研究  

Quantitative study on morphologic features and proliferative activity during DEN-induced hepatocarcinogenesis in rats

作　　者：张新立[1] 史景泉[1] 卞修武[1] 

机构地区：[1]第三军医大学附属西南医院病理学研究所,重庆400038

出　　处：《第三军医大学学报》2001年第3期304-307,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目 !(3 92 70 3 0 3 )

摘　　要：目的　探讨大鼠肝癌变的形态演变和细胞增殖活性的关系。方法　通过DEN诱发启动模型和肝癌模型 ,采用图像分析技术 ,对病变细胞的形态进行测量 ,以PCNA和BrdU免疫组化结果反映细胞增殖活性。结果　形态测量证实卵圆细胞是一大小仅为正常肝细胞的 1 /8、核浆比却大 6倍、形态不规则的增生小细胞 ;增生灶 /结节嗜碱性肝细胞形态参数与肝癌细胞相似 ;PCNA和BrdU阳性细胞主要位于癌前增生灶及肝癌组织中 ;并且病变形态与细胞增殖活性有较好的一致性。Objective To explore the relationship between morphologic evolution and proliferative activity during hepatocarcinogenesis in rats. Methods Imaging analysis technique was used to detect the morphologic parameters of cells in hepatic lesions in both Solt Farber model and diethylnitrosamine (DEN) induced liver cancer model. Immunohistochemistry was employed to detect proliferating cell nuclear antigen (PCNA) and bromodeoxyuridine (BrdU). Results The oval cells were identified as irregular small proliferating cells in size of one eighth of and with a nucleus/cytoplasm ratio of 6 times of the normal hepatocyte by image analysis. The morphometric parameters of basophil hepatocyte in precancerous foci and nodule were similar to those of the liver cancer cell. PCNA and BrdU positive cells were mainly localized within the proliferative foci and hepatocellular carcinoma (HCC) tissues. There was a better consistency between the development of hepatic lesions and cellular proliferative activity. Conclusion The morphologic evolution is closely related to proliferative activity during hepatocarcinogenesis in rats.

关 键 词：肝癌变 细胞增殖活性 增殖细胞核抗原 溴化脱氧尿嘧啶 二乙基亚硝胺 DEN 大鼠 

分 类 号：R735.7[医药卫生—肿瘤]